Bioorganic Chemistry (2021)
Update date:2022-08-17
Topics:
Shan, Yuanyuan
Wang, Jin
Si, Ru
Ma, Yuexiang
Li, Jing
Zhang, Qingqing
Lu, Wen
Zhang, Jie
We previously developed two candidates with potency of inducing vascular normalization, BD7 and B14. However, the definite intracellular molecular target(s) responsible for their activity remains unknown. Herein, we report the discovery and functional assessment of several multifunctional photoaffinity probes for determining the potential biological targets of active compounds. The probes bear a photoaffinity moiety and a bioorthogonal unit attached to B7 or B14 and maintained the bioactivity of the parent active molecules. Using in vitro biological assays, we preliminarily identified VEGFR-2 as a potential intracellular target for the active candidates. Our results demonstrate the utility of these multifunctional photoaffinity probes for analyzing the biological activity and subcellular localization of the intracellular target proteins of active candidates.
View Morewebsite:http://www.sjzthld.com
Contact:86-311-66699812
Address:NO.600 ZHONGSHAN EAST ROAD SHIJIAZHUANG
Contact:+86-570-4336358
Address:No.87 Building,Tianqian,Sidu Town
Qingdao Kylin Trading Co., Ltd.
Contact:0086-532-68979884/58972912/68972263/65/88171519
Address:Room 2308,A building International Trade Center No.230 Changjiang Middle Road of Qingdao Economic Development Zone,Shandong,China.
Changzhou Sunlight Pharmaceutical Co., Ltd.
Contact:+86-519-83131668;83139028;83138042;83137041
Address:JiuliStreet, Benniu Town Changzhou City, Jiangsu Province
Jiangsu Hualun Chemical Industry Co., Ltd
website:http://www.hualunchem.com
Contact:+86-0514-86464168 86507985
Address:39# Middle Renmin Road, Dinghuo Town
Doi:10.1016/S0277-5387(00)86021-0
(1992)Doi:10.1039/C3974000312a
(1974)Doi:10.1016/j.apcata.2018.07.009
(2018)Doi:10.1021/ja00393a005
(1981)Doi:10.1021/jo990631f
(1999)Doi:10.1039/c6nj01721e
(2016)