Tetrahedron Asymmetry p. 2421 - 2428 (2000)
Update date:2022-08-24
Topics:
Wehofsky, Nicole
Haensler, Marion
Kirbach, Sebastian W.
Wissmann, Johannes-Dieter
Bordusa, Frank
The effect of freezing on the enzymatic coupling of highly specific amino acid-containing peptide fragments was investigated using trypsin, α-chymotrypsin, and Bacillus licheniformis Glu-specific endopeptidase as biocatalysts. Comparison with reactions at normal temperature indicates that freezing efficiently represses the cleavage of specific peptide bonds independent of their individual localisation and specificity achieving irreversible and efficient peptide bond formation without proteolytic side reactions. Copyright (C) 2000 Elsevier Science Ltd.
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