Y. Mimaki et al. / Phytochemistry 69 (2008) 729–737
737
1
3
see Table 1; for C NMR (C D N), see Table 2; HRESI–
Kuroda, M., Mimaki, Y., Hasegawa, F., Yokosuka, A., Sashida, Y.,
Sakagami, H., 2001a. Steroidal glycosides from the bulbs of Camassia
leichtlinii and their cytotoxic activities. Chem. Pharm. Bull. 49, 726–
5
5
+
TOFMS m/z: 1007.5375 [M+H]
C H O , 1007.5427).
(calculated for
4
9
83 21
7
31.
Kuroda, M., Mimaki, Y., Yokosuka, A., Sashida, Y., 2001b. Cholestane
glycosides from the bulbs of Galtonia candicans and their cytotoxicity.
Chem. Pharm. Bull. 49, 1042–1046.
3
.13. Acid hydrolysis of 3–7
Kuroda, M., Mimaki, Y., Yokosuka, A., Hasegawa, F., Sashida, Y., 2002.
Cholestane glycosides from the bulbs of Ornithogalum thyrsoides and
their cytotoxic activity against HL-60 leukemia cells. J. Nat. Prod. 65,
1417–1423.
Compounds 3 (4.2 mg), 4 (4.0 mg), 5 (4.8 mg), 6
4.2 mg), and 7 (4.4 mg) were independently subjected to
acid hydrolysis as described for 2 to give aglycones (3a:
(
Kuroda, M., Mimaki, Y., Ori, K., Sakagami, H., Sashida, Y., 2004.
Steroidal glycosides from the bulbs of Ornithogalum thyrsoides. J. Nat.
Prod. 67, 1690–1696.
Mimaki, Y., Kawashima, K., Kanmoto, T., Sashida, Y., 1993. Steroidal
glycosides from Allium albopilosum and A. ostrowskianum. Phyto-
chemistry 34, 799–805.
Mimaki, Y., Kuroda, M., Takaashi, Y., Sashida, Y., 1997. Concinnas-
teoside A, a new bisdesmosidic cholestane glycoside from the stems of
Dracaena concinna. J. Nat. Prod. 60, 1203–1206.
1
2
.4 mg, 0.9 mg and 1.5 mg from 3, 4 and 5, and 6a:
.0 mg and 2.1 mg from 6 and 7, respectively) and sugar
fractions. HPLC analysis of the sugar fractions under the
same conditions as for 1 showed the presence of L-rham-
nose and D-glucose in those of 3 and 4, D-glucose in that
of 5, and L-rhamnose, D-xylose and D-glucose in those of
6
and 7. R (min): 10.61 (D-xylose, positive polarity).
t
Mimaki, Y., Kuroda, M., Kameyama, A., Yokosuka, A., Sashida, Y.,
1
998a. Steroidal constituents of the underground parts of Ruscus
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