3,3'-[(3,3'-Dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)

Base Information

• Chemical Name: 3,3'-[(3,3'-Dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)
• CAS No.: 72-57-1
• Molecular Formula: C34H24N6Na4O14S4
• Molecular Weight: 960.819
• Hs Code.: 32041400
• European Community (EC) Number: 200-786-7
• UN Number: 3077
• NCI Thesaurus Code: C910
• RXCUI: 10878
• Wikipedia: Trypan_blue
• Mol file: 72-57-1.mol

Synonyms:trypan blue(4-);TRYPAN BLUE;5-Amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate;Tetrasodium;(3Z)-5-amino-3-[[4-[4-[(2Z)-2-(8-amino-1-oxo-3,6-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-4-oxonaphthalene-2,7-disulfonate;NCGC00167554-01;72-57-1;CHEBI:78899;BDBM50366943;NCGC00167554-02;NCGC00344528-01;PD163268;3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)

Chemical Property of 3,3'-[(3,3'-Dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)

Chemical Property:

• Appearance/Colour: blueish grey powder
• Melting Point: >300 °C(lit.)
• PSA: 404.26000
• Density: 1.007 g/mL at 20 °C
• LogP: 10.78520
• Storage Temp.: Store at RT.
• Solubility.: H2O: soluble10mg/mL
• Water Solubility.: 10 g/L (25 ºC)
• XLogP3: 4
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 20
• Rotatable Bond Count: 5
• Exact Mass: 868.02333415
• Heavy Atom Count: 58
• Complexity: 1790

Purity/Quality:

98%, ^*data from raw suppliers

TrypanBlue(TechnicalGrade) ^*data from reagent suppliers

Safty Information:

• Pictogram(s): T,Xi
• Hazard Codes: T,Xi
• Statements: 45-41-37/38-36/37/38-22
• Safety Statements: 53-45-36/37/39-36-26

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Dyes -> Benzidine Dyes
• Canonical SMILES: CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O
• Recent NIPH Clinical Trials: Trypan blue staining during ophthalmic surgery
• Uses: Biological stain. Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Technology Process of 3,3'-[(3,3'-Dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)

There total 1 articles about 3,3'-[(3,3'-Dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate) which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

4-amino-5-hydroxy-2,7-naphthalenedisulfonic acid (90-20-0) → trypan blue (72-57-1)

Guidance literature:

DOI:10.1021/ie50192a038

Reference yield:

trypan blue (72-57-1) → o-tolidin (119-93-7) + 3,3'-dimethyl-4-aminobiphenyl (13629-82-8) + 3,3'-dimethyl-4-amino-4'-hydroxybiphenyl (76467-68-0)

Guidance literature:

With sodium dithionate; In various solvent(s); at 70 ℃; pH=6; Further Variations:; Reaction partners; Product distribution;

DOI:10.1191/096032799678845061

upstream raw materials:

4-amino-5-hydroxy-2,7-naphthalenedisulfonic acid

Downstream raw materials:

o-tolidin

3,3'-dimethyl-4-aminobiphenyl

3,3'-dimethyl-4-amino-4'-hydroxybiphenyl