3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride hydrate

Base Information

• Chemical Name: 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride hydrate
• CAS No.: 207738-08-7
• Molecular Formula: C16H20 N2 . 2 Cl H . x H2 O
• Molecular Weight: 331.28056
• Hs Code.: 29215900
• European Community (EC) Number: 688-511-0
• DSSTox Substance ID: DTXSID10597911
• Mol file: 207738-08-7.mol

Synonyms:312693-82-6;3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride hydrate;207738-08-7;3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate;3,3,5,5-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE;4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline;hydrate;dihydrochloride;3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate;MFCD00150104;3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate;TMB (hydrochloride hydrate);DTXSID10597911;KVCWTKDFVVSVSJ-UHFFFAOYSA-N;3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diaminedihydrochloridehydrate;DS-4142;119777-61-6;3,3 inverted exclamation marka,5,5 inverted exclamation marka-Tetramethylbenzidine dihydrochloride hydrate;CS-0015201;CS-0369381;3,3',5,5'-tetramethylbenzidine 2hcl hydrate;O10898;A875981;TETRAMETHYLBENZIDINE HYDRATE DIHYDROCHLORIDE;3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate, 97%;3,3',5,5'-TETRAMETHYL-[1,1'-BIPHENYL]-4,4'-DIAMINE 2HCL HYDRATE;3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate, powder, >=98%;3,3',5,5'-Tetramethyl[1,1'-biphenyl]-4,4'-diamine--hydrogen chloride--water (1/2/1);3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate, Vetec(TM) reagent grade, >=98%

Chemical Property of 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride hydrate

Chemical Property:

• Melting Point: >300oC
• Boiling Point: 459.8oC at 760 mmHg
• Flash Point: 231.9oC
• PSA: 52.04000
• LogP: 6.51800
• Storage Temp.: 2-8°C
• Sensitive.: Moisture & Light Sensitive
• Solubility.: Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 1
• Exact Mass: 330.1265688
• Heavy Atom Count: 21
• Complexity: 226

Purity/Quality:

99% ^*data from raw suppliers

3,3'',5,5''-TetramethylbenzidineDihydrochlorideHydrate(1:2:x) ^*data from reagent suppliers

Safty Information:

• Statements: 22
• Safety Statements: 22-24/25-25-24

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C.O.Cl.Cl
• Description: TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.
• Uses: 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine. Convenient water-soluble form of TMB for determination of peroxidase. 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).