Alexa Fluor 350

Base Information

• Chemical Name: Alexa Fluor 350
• CAS No.: 200554-19-4
• Molecular Formula: C16H14N2O9S
• Molecular Weight: 410.35500
• DSSTox Substance ID: DTXSID201296884
• Nikkaji Number: J1.153.457K
• Wikidata: Q27122727
• Mol file: 200554-19-4.mol

Synonyms:7-amino-4-methyl-6-sulfocoumarin-3-acetic acid;Alexa 350;Alexa Fluor 350;AMCA-S

Chemical Property of Alexa Fluor 350

Chemical Property:

• PKA: 0.46±0.20 Most Basic Temperature: 25°C
• PSA: 182.66000
• Density: 1.74±0.1 g/cm3(Predicted)
• LogP: 1.67990
• Solubility.: Soluble in water, dimethyl sulfoxide, methanol
• XLogP3: -0.6
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 10
• Rotatable Bond Count: 5
• Exact Mass: 410.04200120
• Heavy Atom Count: 28
• Complexity: 857

Purity/Quality:

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

Useful:

• Canonical SMILES: CC1=C(C(=O)OC2=CC(=C(C=C12)S(=O)(=O)O)N)CC(=O)ON3C(=O)CCC3=O

Technology Process of Alexa Fluor 350

There total 3 articles about Alexa Fluor 350 which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 90.0%

O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (105832-38-0) + 7-amino-4-methyl-6-sulfocoumarin-3-acetic acid → 7-amino-3-((((succinimidyl)oxy)carbonyl)methyl)-4-methylcoumarin-6-sulfonic acid (200554-19-4)

Guidance literature:

With pyridine;

DOI:10.1016/S0960-894X(99)00364-9

Reference yield:

7-carboethoxyamido-4-methylcoumarin-3-acetic acid ethyl ester (113721-84-9) → 7-amino-3-((((succinimidyl)oxy)carbonyl)methyl)-4-methylcoumarin-6-sulfonic acid (200554-19-4)

Guidance literature:

Multi-step reaction with 2 steps

1: 30 percent / H₂SO₄ / 70 - 80 °C

2: 90 percent / pyridine

With pyridine; sulfuric acid;

DOI:10.1016/S0960-894X(99)00364-9

Reference yield:

→ 7-amino-3-((((succinimidyl)oxy)carbonyl)methyl)-4-methylcoumarin-6-sulfonic acid (200554-19-4)

Guidance literature:

upstream raw materials:

O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate

7-carboethoxyamido-4-methylcoumarin-3-acetic acid ethyl ester

Refernces

• 1、 -. "Sulfonated derivatives of 7-aminocoumarin". . . Retrieved 2008/06/13.