12627-53-1

Basic information

• Product Name: LEISHMAN'S STAIN
• Synonyms: LEISHMAN'S EOSINE METHYLENE BLUE;LEISHMAN'S EOSINE METHYLENE BLUE SOLUTION;LEISHMAN'S STAIN;LEISHMAN STAIN;LEISHMAN STAIN SOLUTION;METHYLENE BLUE-EOSIN LEISHMAN;EOSIN-POLYCHROME METHYLENE BLUE;EOSIN METHYLENE BLUE ACCORDING TO LEISHMAN
• CAS NO: 12627-53-1
• Molecular Formula: n.a.
• Molecular Weight: 0
• EINECS: 235-732-1
• Mol File: 12627-53-1.mol
• Article Data: 0

Chemical Properties

• Appearance: /Powder
• Storage Temp.: Store at +15°C to +30°C.
• Solubility: methanol: soluble1mg/mL
• Water Solubility: Soluble in water (4 mg/ml), methanol (1 mg/ml), ethanol (4 mg/ml), and methyl cellosolve (20 mg/ml).
• CAS DataBase Reference: LEISHMAN'S STAIN(CAS DataBase Reference)
• NIST Chemistry Reference: LEISHMAN'S STAIN(12627-53-1)
• EPA Substance Registry System: LEISHMAN'S STAIN(12627-53-1)

Safety Data

• Hazard Codes: Xi
• Statements: 36/38-36/37/38
• Safety Statements: 22-24/25-36-26
• WGK Germany: 3
• Hazardous Substances Data: 12627-53-1(Hazardous Substances Data)

Usage

Description

Leishman's Stain is a dark greenish-black powder used as a histological and cytological staining agent. It is a mixture of two dyes, methylene blue and eosin, which work together to provide contrast and color differentiation in various cell types. This staining technique is particularly useful in identifying and differentiating leukocytes, parasites, and other cellular components in blood smears and bone marrow samples.

Uses

Used in Hematology:
Leishman's Stain is used as a staining agent for blood smears to identify and differentiate leukocytes, which are essential for diagnosing various blood disorders and infections.
Used in Tropical Medicine:
In the field of tropical medicine, Leishman's Stain is employed for the identification of parasites and trypanosomas, which are responsible for diseases such as leishmaniasis and trypanosomiasis.
Used in Malaria Detection:
Leishman's Stain acts like a fluorescent stain for the detection of malaria parasites, aiding in the diagnosis and treatment of this widespread tropical disease.
Used in Bone Marrow Staining:
Leishman's Stain is also utilized for staining bone marrow samples, which can help in the identification of abnormal cells and the diagnosis of various hematological disorders.