149194-45-6Relevant articles and documents
Synthesis and evaluation of diphenyl phosphonate esters as inhibitors of the trypsin-like granzymes A and K and mast cell tryptase
Jackson, Delwin S.,Fraser, Stephanie A.,Ni, Li-Ming,Kam, Chih-Min,Winkler, Ulrike,Johnson, David A.,Froelich, Christopher J.,Hudig, Dorothy,Powers, James C.
, p. 2289 - 2301 (2007/10/03)
Thirty-six new amino acid and peptidyl diphenyl phosphonate esters were synthesized and evaluated to identify potent and selective inhibitors for four trypsin-like proteases: lymphocyte granzymes A and K, human mast cell tryptase, and pancreatic trypsin. Among five Cbz derivatives of Lys and Arg homologues, Z-(4-AmPhe)(P)(OPh)2 is the most potent inhibitor for granzyme A, and Z-Lys(P)(OPh)2 is the best inhibitor for granzyme K, mast tryptase, and trypsin. The amidino P1 residue D,L-(4-AmPhGly)(P)(OPh)2 was utilized in a series of compounds with several different N-protecting groups and systematic substitutions at P2 in Cbz-AA derivatives and at P3 in Cbz-AA-Ala derivatives. Generally, these phosphonates inhibit granzyme A and trypsin more potently than granzyme K and tryptase. The P2 Thr and Ala dipeptide phosphonates, Cbz-AA-(4-AmPhGly)(P)(OPh)2, are the most potent inhibitors for granzyme A, and Cbz-Thr-(4-AmPhGly)(P)(OPh)2 (k(obs)[I] = 2220 M-1 s- 1) was quite specific with much lower inhibition rates for granzyme K and trypsin (k(obs)[I] = 3 and 97 M-1 s-1, respectively) and no inhibition with tryptase. The most effective inhibitor of granzyme A was Ph-SO2-Gly- Pro-(4-AmPhGly)(P)(OPh)2 with a second-order rate constant of 3650 M-1 s- 1. The most potent inhibitor for granzyme K was 3,3-diphenylpropanoyl-Pro- (4-AmPhGly)(P)(OPh)2 with a k(obs)/[I] = 1830 M-1 s-1; all other phosphonates inhibited granzyme K weakly (k(obs)/[I] -1 s-1). Human mast cell tryptase was inhibited slowly by these phosphonates with Cbz- Lys(P)(OPh)2 as the best inhibitor (k(obs)/[I] = 89 M-1 s-1). The overall results suggest that scaffolds of Phe-Thr-(4-AmPhe) and Phe-Pro-Lys will be useful to create selective phosphonate inhibitors for granzymes A and K, respectively, and that P4 substituents offer opportunities to further enhance selectivity and reactivity.
