1936-15-8 Usage
Description
Acid Orange 10, also known as Orange G, is an organic sodium salt that is the disodium salt of 7-hydroxy-8-[(E)-phenyldiazenyl]naphthalene-1,3-disulfonic acid. It is characterized by its bright orange color and is available in the form of microcrystals or powder. Acid Orange 10 is soluble in water, slightly soluble in ethanol, and soluble in fiber elements, but insoluble in other organic solvents. It exhibits different color shades when exposed to various chemical conditions, such as strong sulfuric acid, diluted sulfuric acid, nitric acid, and strong hydrochloric acid.
Uses
Used in Histological Staining:
Acid Orange 10 is used as a histological stain, particularly for staining erythrocytes in trichrome methods. It is often combined with other yellow dyes in alcoholic solutions for this purpose.
Used in Connective Tissue Staining:
Acid Orange 10 is used as a collagen stain for connective tissue. It is used in combination with Orcein, Alizarin Blue 2B, and Fast Green FCF in Kornhauser's quadruple stain to highlight the most elementary structures of tissues.
Used in Textile Industry:
Acid Orange 10 is used as a dye for silk and wool products. Its bright orange color and solubility properties make it suitable for dyeing these materials.
Used in Paper and Ink Manufacturing:
Acid Orange 10 is also used in the paper and ink industries for coloring and manufacturing purposes.
Used in Wood and Color Pencil Manufacturing:
The bright orange color of Acid Orange 10 makes it suitable for use in the manufacturing of wood and color pencils.
Used in Biological Staining:
Acid Orange 10 is used for staining and demonstrating cells in the pancreas and pituitary.
Standard:
Acid Orange 10 has been tested for various standards, including light fastness, soaping, perspiration fastness, oxygen bleaching, and fastness to seawater. The results of these tests are as follows:
Light Fastness: ISO 4-5, AATCC 4
Soaping: ISO 1, AATCC 1
Perspiration Fastness: ISO 5, AATCC 1
Oxygen Bleaching: ISO 2, AATCC 2
Fastness to Seawater: ISO 1, AATCC 1
These standards indicate the performance and durability of Acid Orange 10 in various conditions, making it a versatile and reliable dye for various applications.
Preparation
aniline diazo, and 7-Hydroxynaphthalene-1,3-disulfonic acid coupling.
Air & Water Reactions
Azo dyes can be explosive when suspended in air at specific concentrations. This organic acid has a moderate soluble in water. The resulting solutions contain concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid.
Reactivity Profile
Acid Orange 10 is an azo compound. Toxic gases are formed by mixing compounds containing azo groups with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Acid Orange 10 becomes redder and more dull when mixed with copper. Acid Orange 10 is almost destroyed when mixed with iron.
Health Hazard
ACUTE/CHRONIC HAZARDS: When heated to decomposition Acid Orange 10 emits toxic fumes of carbon oxides, nitrogen oxides, sulfur oxides and disodium oxide.
Fire Hazard
Flash point data for Acid Orange 10 are not available; however, Acid Orange 10 is probably combustible.
Biochem/physiol Actions
Orange G is used in collagen stain for connective tissue. It is used with Orcein, Alizarin Blue 2B, and Fast Green FCF in Kornhauser′s quadruple stain for most elementary structures of tissues. It is also used along with acid fuchsin and light green in masson trichrome stain for the staining of cellular structures, red blood cells and muscle cells.
Standard
Light Fastness
Fading
Stain
ISO
4-5
AATCC
4
Purification Methods
Recrystallise this dye from 75% EtOH, dry it for 3hours at 110o and keep it in a vacuum desiccator over H2SO4. The free acid crystallises from EtOH or conc HCl in deep red needles with a green reflex. [Conant & Pratt J Am Chem Soc 48 2483 1923, Drew & Landquist J Chem Soc 292 1938, Beilstein 16 H 301, 16 I 305, 16 II 141, 16 III 327.]
Check Digit Verification of cas no
The CAS Registry Mumber 1936-15-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,9,3 and 6 respectively; the second part has 2 digits, 1 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 1936-15:
(6*1)+(5*9)+(4*3)+(3*6)+(2*1)+(1*5)=88
88 % 10 = 8
So 1936-15-8 is a valid CAS Registry Number.
InChI:InChI=1/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17-;;
