2381-85-3 Usage
Description
Nile Blue Chloride is an organic chloride salt with the chemical structure of 5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium as the cation. It is a fluorescent dye that exhibits bright blue color when dissolved in hot water and ethanol. The dye solution turns orange or brown in strong sulfuric acid and then transforms into a blu-ray to green and finally back to blue upon dilution. When mixed with sodium hydroxide, it forms a red precipitate that is soluble in ethyl ether, resulting in a green fluorescent light brown orange solution. Nile Blue Chloride is also a potent photosensitizer for photodynamic therapy.
Uses
Used in Histology and Lipid Staining:
Nile Blue Chloride is used as a histological and lipid stain due to its ability to bind to lipids and provide a distinct blue coloration. This property makes it useful for visualizing and identifying lipid-containing structures in biological samples.
Used in Photodynamic Therapy:
As a potent photosensitizer, Nile Blue Chloride is used in photodynamic therapy for its ability to generate reactive oxygen species upon light activation. This application is particularly relevant in the treatment of certain medical conditions, such as cancer, where the generation of reactive oxygen species can lead to the destruction of targeted cells.
Used in Textile Industry:
Nile Blue Chloride is used in the textile industry as a dye for cotton, providing good light fastness, perspiration fastness, ironing fastness, and soaping properties. The dye is known for its fading and staining characteristics, which are rated as follows:
Light Fastness: A
Perspiration Fastness: 1
Ironing Fastness: 2-3
Soaping: 3
Fading: 1
Stain: 1
These ratings indicate that Nile Blue Chloride is a suitable dye for cotton fabrics, offering good resistance to light exposure and ironing while maintaining its color and appearance even after washing and exposure to sweat.
Preparation
commonly known as the Nile Blue. 5-(Diethylamino)-2-nitrosophenol?and Naphthalen-1-amine hydrochloride reaction.
Standard( Cotton )
Light Fastness
Fading
Stain
Check Digit Verification of cas no
The CAS Registry Mumber 2381-85-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,3,8 and 1 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 2381-85:
(6*2)+(5*3)+(4*8)+(3*1)+(2*8)+(1*5)=83
83 % 10 = 3
So 2381-85-3 is a valid CAS Registry Number.
InChI:InChI=1/C20H20N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12H,3-4,21H2,1-2H3;1H/q+1;/p-1
2381-85-3Relevant articles and documents
Transition-Metal-Mediated versus Tetrazine-Triggered Bioorthogonal Release Reactions: Direct Comparison and Combinations Thereof
Dzijak, Rastislav,Mancuso, Francesca,Mertlíková-Kaiserová, Helena,Rahm, Michal,Vrabel, Milan
, p. 1669 - 1675 (2020)
Bioorthogonal cleavage reactions are gaining popularity in chemically inducible prodrug activation and in the control of biomolecular functions. Despite similar applications, these reactions were developed and optimized on different substrates and under different experimental conditions. Reported herein is a side-by-side comparison of palladium-, ruthenium- and tetrazine-triggered release reactions, which aims at comparing the reaction kinetics, efficiency and overall advantages and limitations of the methods. In addition, we disclose the possibility of mutual combination of the cleavage reactions. Finally, we compare the efficiency of the bioorthogonal deprotections in cellular experiments, which revealed that among the three methods investigated, the palladium- and the tetrazine-promoted reaction can be used for efficient prodrug activation, but only the tetrazine-triggered reactions proceed efficiently inside cells.
