2513-33-9Relevant articles and documents
Ratiometric Visualization of NO/H2S Cross-Talk in Living Cells and Tissues Using a Nitroxyl-Responsive Two-Photon Fluorescence Probe
Zhou, Yibo,Zhang, Xiufang,Yang, Sheng,Li, Yuan,Qing, Zhihe,Zheng, Jing,Li, Jishan,Yang, Ronghua
, p. 4587 - 4594 (2017)
It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S) because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit. The newly proposed probe has been successfully applied in ratiometric two-photon bioimaging of endogenous HNO derived from NO and H2S interaction in the human umbilical vein cells (HUVECs) and as well as in rat brain tissues. Intriguingly, the imaging results consistently demonstrate that the mutually dependent upgeneration of H2S and NO are present in living biosystems, indicating that this molecular probe would provide a powerful approach to elucidate the chemical foundation for the anfractuous cross-talk between the NO and H2S signaling pathways in biology.
Ratiometric and colorimetric detection of hydrogen sulfide with high selectivity and sensitivity using a novel FRET-based fluorescence probe
Huang, Kunzhu,Liu, Meihui,Liu, Zhiguo,Cao, Dongsheng,Hou, Jing,Zeng, Wenbin
, p. 88 - 94 (2015)
Abstract With recognition of the biological significance of H2S, we have designed and synthesized a novel ratiometric and colorimetric fluorescence probe, CR-DNP based on a H2S induced thiolysis reaction as well as the fluorescence resonance energy transfer process. It displayed some desirable properties, such as good photostability, high selectivity and excellent sensitivity. Also, a considerably fluorescence enhancement (15-fold) and distinct visible color change (colorless to bright orange) was observed. A good linear relationship between the emission intensity ratios and NaHS concentrations suggested that it can detect H2S quantitatively with a detection limit as low as 0.4 μM. Furthermore, due to its biocompatibility, the sensor could easily permeate through the cell membrane and trace the intracellular H2S levels change.
A FRET-based ratiometric fluorescent probe for highly selective detection of cysteine based on a coumarin-rhodol derivative
Bai, Yu,Wu, Ming-Xia,Ma, Qiu-Juan,Wang, Chun-Yan,Sun, Jing-Guo,Tian, Mei-Ju,Li, Jian-Sheng
, p. 14763 - 14771 (2019)
Cysteine, as an important amino acid in the human body, plays a vital role in people's normal life activities. In this paper, a ratiometric fluorescent probe for detecting cysteine was designed and synthesized based on the fluorescence resonance energy transfer (FRET) process. In this FRET system, a coumarin derivative was used as the energy donor, a rhodol fluorophore was chosen as the energy receptor, and an acrylate group was utilized as a cysteine recognition unit. In the absence of cysteine, the rhodol receptor was in the non-fluorescent lactone state and the FRET process was inhibited. Upon addition of cysteine, the closed spirolactone form was converted to a conjugated fluorescent xanthene form to induce the occurrence of FRET which resulted in a fluorescent signal decrease at 470 nm and enhancement at 543 nm. The ratiometric fluorescent probe exhibited excellent selectivity to Cys over Hcy and GSH. In addition, I543nm/I470nm of the probe for cysteine displayed a good linear relationship in the range of 5.0 × 10-7-1.0 × 10-4 mol L-1, and the detection limit was 2.0 × 10-7 mol L-1. Furthermore, the probe showed low cell toxicity and was successfully applied to the confocal imaging of cysteine in HepG2 cells using dual emission channels.
Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation
Ou, Pan,Zhang, Ruilong,Liu, Zhengjie,Tian, Xiaohe,Han, Guangmei,Liu, Bianhua,Hu, Zhangjun,Zhang, Zhongping
supporting information, p. 2261 - 2265 (2019/01/29)
Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the re
