27274-31-3Relevant articles and documents
Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayers
Paulick, Margot G.,Wise, Amber R.,Forstner, Martin B.,Groves, Jay T.,Bertozzi, Carolyn R.
, p. 11543 - 11550 (2007)
Positioned at the C-terminus of many eukaryotic proteins, the glycosylphosphatidylinositol (GPI) anchor is a posttranslational modification that anchors the modified proteins in the outer leaflet of the plasma membrane. GPI-anchored proteins play vital ro
Non-natural amino acid and application thereof Recombinant protein and recombinant protein conjugate comprising same
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Paragraph 0094; 0098; 0099-0100, (2021/10/27)
The invention provides a non-natural amino acid. A compound represented by formula (I) or an enantiomer thereof. The invention also provides application of the non-natural amino acid. Further, the present invention also provides a protein conjugate comprising the recombinant protein and of the non-natural amino acid prepared from the recombinant protein. The non-natural amino acid provided by the invention is simple and convenient to prepare, good in safety, not prone to inactivation when inserted into a protein, high in coupling ratio with a coupling part, good in stability of the obtained conjugate, and capable of being applied to various fields, especially in preparation of recombinant protein or recombinant protein conjugate.
Stereoselective Synthesis of Molecular Square and Granny Knots
Leigh, David A.,Pirvu, Lucian,Schaufelberger, Fredrik
supporting information, p. 6054 - 6059 (2019/04/26)
We report on the stereoselective synthesis of both molecular granny and square knots through the use of lanthanide-complexed overhand knots of specific handedness as three-crossing "entanglement synthons". The composite knots are assembled by combining two entanglement synthons (of the same chirality for a granny knot; of opposite handedness for a square knot) in three synthetic steps: first, a CuAAC reaction joins together one end of each overhand knot. Ring-closing olefin metathesis (RCM) then affords the closed-loop knot, locking the topology. This allows the lanthanide ions necessary for stabilizing the entangled conformation of the synthons to subsequently be removed. The composite knots were characterized by 1H and 13C NMR spectroscopy and mass spectrometry and the chirality of the knot stereoisomers compared by circular dichroism. The synthetic strategy of combining building blocks of defined stereochemistry (here overhand knots of λ- or Δ-handed entanglement) is reminiscent of the chiron approach of using minimalist chiral synthons in the stereoselective synthesis of molecules with multiple asymmetric centers.
