2800-80-8 Usage
Description
Bromophenol Red is an organic compound with the chemical formula C??H??BrO?S and the CAS number 2800-80-8. It is a synthetic dye that exhibits a red color and is commonly used in various applications due to its unique properties.
Uses
Used in Molecular Biology:
Bromophenol Red is used as a luminol signal co-enhancer in the chemiluminescent detection of sequence-specific DNA. Its role in this application is to improve the sensitivity and accuracy of DNA detection methods, making it a valuable tool in molecular biology research.
Used in Enzyme Inhibition:
Bromophenol Red is also used as an inhibitor of glucose 6-phosphate dehydrogenase (G6PD), an enzyme involved in the pentose phosphate pathway. By inhibiting this enzyme, Bromophenol Red can be utilized in studying the effects of G6PD inhibition on cellular processes and understanding the role of G6PD in various biological functions.
Check Digit Verification of cas no
The CAS Registry Mumber 2800-80-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,8,0 and 0 respectively; the second part has 2 digits, 8 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 2800-80:
(6*2)+(5*8)+(4*0)+(3*0)+(2*8)+(1*0)=68
68 % 10 = 8
So 2800-80-8 is a valid CAS Registry Number.
InChI:InChI=1/C19H12Br2O5S/c20-14-9-11(5-7-16(14)22)19(12-6-8-17(23)15(21)10-12)13-3-1-2-4-18(13)27(24,25)26-19/h1-10,22-23H
2800-80-8Relevant articles and documents
Chemical bromination of phenol red by hydrogen peroxide is possible in the absence of haloperoxidases
Terron, Maria C.,Verhagen, Frank J.M.,Franssen, Maurice C.R.,Field, Jim A.
, p. 1445 - 1452 (2007/10/03)
The dye phenol red (phenolsulphonphthalein) was chemically brominated to bromophenol blue (3',3',5',5'-tetrabromophenolsulphonphthalein) directly by hydrogen peroxide (20 mM or higher) under acidic pH (0.5-4.5) and moderate temperature (30°C). Since this bromination reaction takes place in the absence of any chemical or biological catalyst, the results represent an important warning concerning a commonly used assay for detecting or screening haloperoxidases. Bromophenol red (5',5'-dibromophenolsulphonphthalein) and another unidentified compound were observed as temporary intermediates. Incubation of the reaction mixture with KCl instead of NaBr did not yield any chlorinated products under the same conditions.
