286949-85-7Relevant articles and documents
Synthesis and evaluation of vitamin D-based cationic lipids for gene delivery in vitro
Ren, Tan,Zhang, Guisheng,Liu, Feng,Liu, Dexi
, p. 891 - 894 (2007/10/03)
A new panel of steroidal cationic lipids has been synthesized for gene delivery. Using commercially available vitamin D2 (calciferol) or vitamin D3 (cholecalciferol) as hydrophobic motifs and a variety of cationic head groups as binding sites for negatively charged phosphate groups in DNA, we demonstrated that the transfection activity of the synthetic vitamin D-based cationic lipids 1d, 2d formulated with dioleoylphosphatidylethanolamine (DOPE) as a co-lipid is comparable to that of 3-(-[N-N',N'-dimethylaminoethane)carbamoyl]cholesterol (DC-Chol). These synthetic lipids are effective in transfecting a variety of cell lines. These results suggest that vitamin D-based cationic lipids are useful transfection reagents for in vitro gene transfer studies. (C) 2000 Published by Elsevier Science Ltd.