516-59-6

Basic information

• Product Name: 5A-PREGNAN-20A-OL-3-ONE
• Synonyms: 5A-PREGNAN-20A-OL-3-ONE;allopregnan-20 alpha-ol-3-one;(20S)-20-Hydroxy-5α-pregnan-3-one;Allopregnan-20a-ol-3-one;5α-Pregnane-3-one-20α-ol;20α-Hydroxy-5α-pregnan-3-one;(5S,8R,9S,10S,13S,14S,17S)-17-[(1S)-1-hydroxyethyl]-10,13-dimethyl-1,2,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-3-one
• CAS NO: 516-59-6
• Molecular Formula: C₂₁H₃₄O₂
• Molecular Weight: 318.49346
• Mol File: 516-59-6.mol
• Article Data: 1

Chemical Properties

• Melting Point: 179°
• Boiling Point: 433.8°Cat760mmHg
• Flash Point: 185°C
• Appearance: /
• Density: 1.048g/cm³
• Vapor Pressure: 2.4E-09mmHg at 25°C
• Refractive Index: 1.522
• Storage Temp.: -20°C Freezer
• Solubility: Chloroform (Slightly), Ethanol (Slightly), Methanol (Slightly)
• CAS DataBase Reference: 5A-PREGNAN-20A-OL-3-ONE(CAS DataBase Reference)
• NIST Chemistry Reference: 5A-PREGNAN-20A-OL-3-ONE(516-59-6)
• EPA Substance Registry System: 5A-PREGNAN-20A-OL-3-ONE(516-59-6)

Safety Data

• Hazardous Substances Data: 516-59-6(Hazardous Substances Data)

Usage

Description

5α-Pregnane-3-one-20α-ol, also known as 5A-PREGNAN-20A-OL-3-ONE, is a steroid metabolite derived from the metabolism of progesterone in adipose cells. It is a significant component of the steroid metabolome and has been identified for its potential use in various applications, particularly in the field of medical research and diagnostics.

Uses

Used in Medical Research:
5A-PREGNAN-20A-OL-3-ONE is used as a research tool for studying its potential role in the laboratory diagnosis of schizophrenia. 5A-PREGNAN-20A-OL-3-ONE is investigated for its association with the disease's pathology and its ability to serve as a biomarker for the disorder.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 5A-PREGNAN-20A-OL-3-ONE is used as a metabolite of progesterone, which can be further explored for the development of new drugs targeting hormonal imbalances and related health conditions. Its understanding can contribute to the advancement of treatments for various disorders influenced by progesterone levels.
Used in Diagnostic Applications:
5A-PREGNAN-20A-OL-3-ONE is utilized as a diagnostic marker in the detection and monitoring of schizophrenia. Its presence in the steroid metabolome can provide valuable information for clinicians to assess the disease's progression and the effectiveness of treatments.

InChI:InChI=1/C21H34O2/c1-13(22)17-6-7-18-16-5-4-14-12-15(23)8-10-20(14,2)19(16)9-11-21(17,18)3/h13-14,16-19,22H,4-12H2,1-3H3/t13-,14-,16-,17+,18-,19-,20-,21+/m0/s1

Upstream product

• 18003-79-7 3,3-dimethoxy-5α-pregnan-20-one 
• 474-45-3 (3α,5α)-3-aminopregnan-20-one 
• 566-65-4 dihydroprogesterone 
• 6003-18-5 3α,20α_F-diacetoxy-5α-pregnane 
• 118490-26-9 20α_F-acetoxy-5α-pregnan-3β-ol 
• 6003-19-6 20α_F-acetoxy-5α-pregnan-3α-ol 
• 55547-49-4 acetic acid-(3β-hydroxy-pregnen-(5)-yl-(20α_F)-ester) 
• 6010-88-4 20α_F-acetoxy-5α-pregnan-3-one 
• 474-44-2 funtumidine 

Downstream Products

• 432552-71-1 (20S)-3,3-difluoro-5α-pregnan-20-yl benzoate 
• 432552-69-7 (20S)-3-oxo-5α-pregnan-20-yl benzoate 

Relevant articles and documents

Rabbit 3-hydroxyhexobarbital dehydrogenase is a NADPH-preferring reductase with broad substrate specificity for ketosteroids, prostaglandin D2, and other endogenous and xenobiotic carbonyl compounds

3-Hydroxyhexobarbital dehydrogenase (3HBD) catalyzes NAD(P) +-linked oxidation of 3-hydroxyhexobarbital into 3-oxohexobarbital. The enzyme has been thought to act as a dehydrogenase for xenobiotic alcohols and some hydroxysteroids, but its physiological function remains unknown. We have purified rabbit 3HBD, isolated its cDNA, and examined its specificity for coenzymes and substrates, reaction directionality and tissue distribution. 3HBD is a member (AKR1C29) of the aldo-keto reductase (AKR) superfamily, and exhibited high preference for NADP(H) over NAD(H) at a physiological pH of 7.4. In the NADPH-linked reduction, 3HBD showed broad substrate specificity for a variety of quinones, ketones and aldehydes, including 3-, 17- and 20-ketosteroids and prostaglandin D2, which were converted to 3α-, 17β- and 20α-hydroxysteroids and 9α,11β- prostaglandin F2, respectively. Especially, α-diketones (such as isatin and diacetyl) and lipid peroxidation-derived aldehydes (such as 4-oxo- and 4-hydroxy-2-nonenals) were excellent substrates showing low Km values (0.1-5.9 μM). In 3HBD-overexpressed cells, 3-oxohexobarbital and 5β-androstan-3α-ol-17-one were metabolized into 3-hydroxyhexobarbital and 5β-androstane-3α,17β-diol, respectively, but the reverse reactions did not proceed. The overexpression of the enzyme in the cells decreased the cytotoxicity of 4-oxo-2-nonenal. The mRNA for 3HBD was ubiquitously expressed in rabbit tissues. The results suggest that 3HBD is an NADPH-preferring reductase, and plays roles in the metabolisms of steroids, prostaglandin D2, carbohydrates and xenobiotics, as well as a defense system, protecting against reactive carbonyl compounds.