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531-52-2

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531-52-2 Usage

Description

Triphenylformazan, also known as 1,3,5-triphenyltetrazolium formazan, is a chemical compound that is widely used in various applications due to its unique properties. It is a red crystalline solid that is soluble in water and has a molecular weight of 287.3 g/mol. Triphenylformazan is known for its ability to form a colored formazan product when reduced, making it a useful indicator in various assays and tests.

Uses

Used in Germination and Viability Tests:
Triphenylformazan is used as an indicator in germination and viability tests to assess the metabolic activity of cells. It is reduced to a colored formazan product, which can be measured spectrophotometrically, providing a quantitative assessment of cell viability and germination potential.
Used in Quantification of TTC Reduction Activity:
In the field of microbiology, Triphenylformazan is used as a standard to study the optical density of formazan produced by 2,3,5-triphenyltetrazolium chloride (TTC)-treated bacterial cells. This allows for the quantification of TTC reduction activity, which is an important parameter in assessing bacterial metabolism and growth.
Used in Seed Testing:
Triphenylformazan is used as a standard in seed testing to evaluate the germination potential of seeds. It is used to study the optical density of formazan produced by TTC-treated milled seeds, providing a quantitative measure of seed viability and germination capacity.
Used in Cardiology Research:
In cardiology research, Triphenylformazan is used to stain heart tissue for infarct size assessment. It can be used in conjunction with perfusion fixation to allow morphometric studies, in addition to histology and immunohistochemistry, providing valuable insights into the extent of tissue damage and the effectiveness of potential treatments.

Check Digit Verification of cas no

The CAS Registry Mumber 531-52-2 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,3 and 1 respectively; the second part has 2 digits, 5 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 531-52:
(5*5)+(4*3)+(3*1)+(2*5)+(1*2)=52
52 % 10 = 2
So 531-52-2 is a valid CAS Registry Number.
InChI:InChI=1/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+

531-52-2SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 12, 2017

Revision Date: Aug 12, 2017

1.Identification

1.1 GHS Product identifier

Product name Tetrazole Red Formazan

1.2 Other means of identification

Product number -
Other names 1,3,5-Triphenyltetrazolium formazan

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:531-52-2 SDS

531-52-2Relevant articles and documents

Electron spin resonance signals of tetrazolium compounds.

Lofberg

, p. 503 - 504 (1965)

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Synthesis and Electrochemical Properties of 2-(4-R1-Phenyl)-6-(4-R2-phenyl)-4-phenyl-3,4-dihydro1,2,4,5-tetrazin-1(2H)-yls

Akhmatova, A. A.,Balandina, A. V.,Chernyaeva, O. Yu.,Kostryukov, S. G.,Kozlov, A. Sh.,Kraynov, E. V.,Lukshina, Yu. I.,Pryanichnikova, M. K.

, p. 341 - 351 (2020/04/27)

Abstract: A new methodology for creating electroactive components for organic batteries,based on the construction of a molecular platform including stable3,4-dihydro-1,2,4,5-tetrazin-1(2H)-ylradicals was described. A series of2-(4-R1-phenyl)-6-(4-R2-phenyl)-4-phenyl-3,4-dihydro-1,2,4,5-tetrazin-1(2H)-yls with substituents of various nature wasobtained. It was shown that the substituents R1 inthe aromatic ring at position 2 of the tetrazinyl fragment influence the valueof the oxidation potential in the radical, but do not influence the value of thereduction potentials, while the substituent R2 of thearomatic ring at position 6 influence the values of the reduction potentials andpractically do not influence oxidation potential values. Based on the obtainedelectrochemical data, a correlation structure–potential value was revealed forthe cathodic and anodic process, with the help of which triarylsubstituted3,4-dihydro-1,2,4,5-tetrazin-1(2H)-ylradicals with high values of the electrochemical gap were obtained.

Method for preparing chlorinated-3-substituted-2,5-diphenyl tetrazole under catalysis of copper Lewis acid surfactant

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Paragraph 0057; 0060; 0061; 0062; 0063, (2018/09/12)

The invention belongs to the technical field of organic synthesis and specifically relates to a method for preparing chlorinated-3-substituted-2,5-diphenyl tetrazole under the catalysis of a copper Lewis acid surfactant. The method comprises the following steps: 1) reacting benzaldehyde, phenylhydrazine, arylamine and sodium nitrite in solvent water by taking a copper Lewis acid surfactant Cu(OSO2CnH2n+1)2 as a catalyst to obtain 3-aryl-2,5-diphenylformazan; and 2) cyclizing and chlorinating 3-aryl-2,5-diphenylformazan in a mixed solvent of dichloromethane and water under the condition of a solid chlorination reagent N-chlorosuccinimide to obtain chlorinated-3-substituted-2,5-diphenyl tetrazole. By using the method, three-step conversion is completed according to a ''one-pot method'' continuous strategy, not only is an intermediate separation step omitted, but also the purification step is simple as well as convenient and easy to operate.

Microwave mediated solvent free synthesis of formazans catalyzed by simple ionic liquids derived from dialkylammonium salts

Das, Pranab Jyoti,Begum, Jesmin

, p. 44604 - 44609 (2015/06/02)

A microwave mediated, ionic liquid catalyzed, VOC free and one pot synthesis of formazans was developed. In an alternative procedure, resin immobilized diazonium ions was used as a solid supported reaction for formazan synthesis. The efficiency of both the procedures was examined with respect to yield of product, reduction of reaction time and environmental impact. Products were obtained in a short reaction time and in moderate to high yield. This study was undertaken to find an alternative green protocol for the synthesis of formazans using ionic liquid as catalyst in aqueous media in the absence of corrosive mineral acids, buffered solutions and VOCs.

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