548-24-3 Usage
Description
EOSIN B is a synthetic dye belonging to the xanthene class, characterized by its red fluorescent color and acidic properties. It is commonly used as a histological stain and in dyeing applications for various materials.
Uses
Used in Textile Industry:
EOSIN B is used as a dye for wool, cotton, and paper, providing vibrant coloration and enhancing the appearance of these materials.
Used in Histology:
EOSIN B is used as a stain for epithelia, muscular fibers, and nuclei, aiding in the visualization and differentiation of these structures in histological samples.
Used in Cell and Tissue Staining:
EOSIN B sodium salt is used as a cell and tissue stain, specifically for staining cell granules, nuclei, and microorganisms. It is also used as a counter stain for collagen and as a stain for cell differentiation of the anterior pituitary when coupled with Azure A.
Preparation
will 4′,5′-Dibromofluorescein?(C.I. Acid Orange 11, C.I. 45370) nitrification, and translated into sodium salt or ammonium salt.
Purification Methods
Free it from inorganic halides by repeated crystallisation from butan-1-ol. [Beilstein 19/6 V 469.]
Standard
Light Fastness
Fading
Stain
ISO
3
Check Digit Verification of cas no
The CAS Registry Mumber 548-24-3 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,4 and 8 respectively; the second part has 2 digits, 2 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 548-24:
(5*5)+(4*4)+(3*8)+(2*2)+(1*4)=73
73 % 10 = 3
So 548-24-3 is a valid CAS Registry Number.
InChI:InChI=1/C20H8Br2N2O9.2Na/c21-13-15(25)11(23(28)29)5-9-17(13)32-18-10(6-12(24(30)31)16(26)14(18)22)20(9)8-4-2-1-3-7(8)19(27)33-20;;/h1-6,25-26H;;/q;2*+1/p-2