57160-56-2Relevant articles and documents
Development of a Class-Specific Competitive ELISa for the Benzoylphenylurea Insecticides
Wang, Shuo,Allan, Robin D.,Skerritt, John H.,Kennedy, Ivan R.
, p. 3330 - 3338 (2007/10/03)
A competitive enzyme-linked immunosorbert assay (ELISA) was developed for the quantitative detection of five benzophenylurea insecticides in soil (90% methanol extraction) and water. No significant matrix effects were observed. Conjugates of diflubenzuron derivatives with proteins were prepared by attachment at either the urea nitrogen of diflubenzuron or the 4-position of the aniline ring, wherein bridging groups of various lengths were used between the hapten and the protein carrier. All combinations of antiserum to each immunogen and enzyme tracer were examined, and an assay was developed using an antibody developed to a hapten conjugate coupled through a succinyl chain at the 4-aniline position. The assay gave 50% inhibition of antibody binding (IC50) at 0.6 ppb for diflubenzuron, 5 ppb for teflubenzuron, 10 ppb for flufenoxuron, 31 ppb for lufenuron, and 45 ppb for chlorfluazuron, with detection limits in the range of 0.05-2.3 ppb for these compounds. The basis for the superior cross-reaction of diflubenzuron was examined by molecular modeling, which suggested that the planarity of the molecules, electron-withdrawing groups, and steric effects of chlorines attached to the phenyl ring may be critical factors affecting antibody binding.
