80265-04-9 Usage
Description
4-METHYLUMBELLIFERYL-2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE, also known as 4-MU-GlcNAc, is a synthetic compound that serves as a fluorogenic substrate. It is commonly used in biochemical assays to measure the activity of enzymes, specifically N-Acetyl-α-D-glucosaminidase. 4-METHYLUMBELLIFERYL-2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE is characterized by its ability to emit fluorescence upon enzymatic cleavage, making it a valuable tool in research and diagnostic applications.
Uses
Used in Enzyme Activity Assays:
4-METHYLUMBELLIFERYL-2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE is used as a fluorogenic substrate for assays of N-Acetyl-α-D-glucosaminidase. It allows researchers to monitor enzyme activity through the increase in fluorescence, providing a sensitive and quantitative method for studying enzyme function and inhibition.
Used in Sanfilippo B Syndrome Research and Diagnosis:
In the field of medical research and diagnostics, 4-METHYLUMBELLIFERYL-2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE is used for Sanfilippo B Syndrome. This is a rare genetic disorder characterized by a deficiency in the enzyme N-Acetyl-α-D-glucosaminidase, which is responsible for breaking down heparan sulfate, a complex sugar found in connective tissues. By using 4-MU-GlcNAc as a substrate, researchers and clinicians can assess the activity of the enzyme and diagnose the syndrome, as well as evaluate the effectiveness of potential treatments.
Check Digit Verification of cas no
The CAS Registry Mumber 80265-04-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,0,2,6 and 5 respectively; the second part has 2 digits, 0 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 80265-04:
(7*8)+(6*0)+(5*2)+(4*6)+(3*5)+(2*0)+(1*4)=109
109 % 10 = 9
So 80265-04-9 is a valid CAS Registry Number.
80265-04-9Relevant articles and documents
4-METHYLUMBELLIFERYL 2-ACETAMIDO-2-DEOXY-α-D-GLUCOPYRANOSIDE, A FLUOROGENIC SUBSTRATE FOR N-ACETYL-α-D-GLUCOSAMINIDASE
Chow, Philip,Weissmann, Bernard
, p. 87 - 94 (1981)
Condensation of dimeric 3,4,6-tri-O-acetyl-2-deoxy-2-nitroso-α-D-glucopyranosyl chloride with 4-methylumbelliferone gave crystalline 4-methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-oximino-α-D-arabino-hexopyranoside.Acetylation of this adduct, reduction of the resulting crude O-acetyloxime with borane in oxolane, and acetylation gave the 3,4,6-tri-O-acetyl derivative of 4-methylumbelliferyl 2-acetamido-2-deoxy-α-D-glucopyranoside (1).A new sensitive assay of N-acetyl-α-D-glucosaminidase (EC 3.2.1.50) is made possible by fluorometric measurement of 4-methylumbelliferone liberated by enzymic hydrolysis of glycoside 1.Such assays are illustrated by results obtained with enzyme preparations from pig liver and human-blood serum.
