9028-76-6 Usage
Description
Cholesterol oxidase is a bacterial-specific flavoenzyme produced from Streptomyces sp, with a molecular weight of 62 kDa. It has a pH and temperature optimum of 7 and 37 °C, respectively, and comprises a FAD-binding and a steroid-binding domain. This enzyme catalyzes the oxidation and isomerization of steroids containing a 3β hydroxyl group and a double bond at the 5–6 of the steroid ring system. Cholesterol oxidase is a member of a large family of flavin-specific oxidoreductases and is found in two different forms: one where the flavin adenine dinucleotide (FAD) cofactor is covalently linked to the protein and one where the cofactor is non-covalently bound to the protein.
Uses
Cholesterol oxidase has a number of important applications across various industries:
Used in Medical Industry:
Cholesterol oxidase is used as a diagnostic tool for determining serum cholesterol levels, which is critical for the assessment of a variety of diseases including hypercholesterolemia, coronary heart disease, and lipid disorders for estimating the risk of thrombosis and myocardial infarction. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids.
Used in Research:
Cholesterol oxidase has been used in studies to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. It has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.
Used in Food Industry:
Cholesterol oxidase is used for determining cholesterol levels in food products, which helps in assessing the health implications of consuming these products.
Used in Agricultural Industry:
Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil, which can help in reducing crop damage and improving agricultural productivity.
Used in Pharmaceutical Industry:
Cholesterol oxidase has been used as a molecular probe to elucidate cellular membrane structures, which can aid in the development of new drugs and therapies targeting membrane-related diseases.
Biochem/physiol Actions
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.
Check Digit Verification of cas no
The CAS Registry Mumber 9028-76-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,2 and 8 respectively; the second part has 2 digits, 7 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 9028-76:
(6*9)+(5*0)+(4*2)+(3*8)+(2*7)+(1*6)=106
106 % 10 = 6
So 9028-76-6 is a valid CAS Registry Number.
