91254-23-8Relevant articles and documents
Kinetic Isotope Effect in the Metabolic Demethylation of Temazepam
Maksay, Gabor,Tegyey, Zsuzsanna,Oetvoes, Laszlo
, p. 57 - 60 (2007/10/02)
The kinetics of the metabolic demethylation of temazepam were examined.Oxidative cleavage of the 1-methyl C-H bonds is rate-determining in the 13000 g supernatant of mouse liver homogenate, as shown by the primary deuterium isotope effect (3.6) for demethylation of 1-CD3-labelled temazepam.With a reduced supply of NADPH, reduction of the cytochrome P-450-substrate complex becomes rate-limiting.Decomposition of the 1-N-hydroxymethyl intermediate is fast enough not to influence the rate of demethylation.