92-32-0 Usage
Description
PYRONIN Y is a cationic dye that intercalates RNA and is used as a bacterial and biological stain. It is visible as a red band during electrophoresis and has been shown to accumulate in the mitochondria of viable cells. PYRONIN Y can be used in conjunction with Methyl Green or Nancy-520 as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Uses
Used in Biotechnology Industry:
PYRONIN Y is used as a fluorescent differential stain for nucleic acids in paraffin tissue sections, in conjunction with Methyl Green or Nancy-520.
Used in Hematopoietic Stem Cell Research:
PYRONIN Y is used as a component of the polychlorinated biphenyls (PCBs) solution for staining mammary gland side population cells (MG-SPs) and staining of CD34+ cells.
Used in Bone Marrow Cell Staining:
PYRONIN Y is used in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs).
Biochem/physiol Actions
Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.
Purification Methods
Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]
Check Digit Verification of cas no
The CAS Registry Mumber 92-32-0 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 9 and 2 respectively; the second part has 2 digits, 3 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 92-32:
(4*9)+(3*2)+(2*3)+(1*2)=50
50 % 10 = 0
So 92-32-0 is a valid CAS Registry Number.
InChI:InChI=1/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
92-32-0Relevant articles and documents
Supramolecular chemistry of pyronines B and Y, β-cyclodextrin and linked β-cyclodextrin dimers
Ngo, Huy T.,Clements, Philip,Easton, Christopher J.,Pham, Duc-Truc,Lincoln, Stephen F.
experimental part, p. 687 - 692 (2010/08/22)
The complexation of cationic pyronine B(PB+) and pyronine Y(PY+) by β-cyclodextrin (CD) and two linked βCD dimers, N,N′-bis((2AS,3AS)-3A-deoxy-β- cyclodextrin-3A-yl)succinamide, 33βCD2
