98001-69-5

Basic information

• Product Name: protamine sulfate
• Synonyms: PROTAMINE SULFATE
• CAS NO: 98001-69-5
• Molecular Formula: C264H460 N128 O65 S7
• Molecular Weight: 0
• Mol File: 98001-69-5.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: 2-8°C
• CAS DataBase Reference: protamine sulfate(CAS DataBase Reference)
• NIST Chemistry Reference: protamine sulfate(98001-69-5)
• EPA Substance Registry System: protamine sulfate(98001-69-5)

Safety Data

• WGK Germany: 3
• RTECS: VO9470000
• Hazardous Substances Data: 98001-69-5(Hazardous Substances Data)

Usage

Description

Protamine sulfate is a highly basic protein that is isolated from fish sperm and has the ability to bind to nucleic acids, particularly DNA and RNA. It is known for its use in various applications in the field of molecular biology and medicine.

Uses

Used in Molecular Biology:
Protamine sulfate is used as a transfection agent for delivering genetic material into cells. It forms stable complexes with nucleic acids, protecting them from degradation and facilitating their uptake by target cells.
Used in Gene Therapy:
Protamine sulfate is used as a carrier for nucleic acids in gene therapy applications. It helps in the efficient delivery of therapeutic genes to target cells, enhancing the therapeutic potential of gene therapy.
Used in Stem Cell Research:
Protamine sulfate is used as a transfection agent for delivering shRNA into mesenchymal stem cells (MSC) and other types of stem cells. This allows for the manipulation of gene expression in these cells, which is crucial for understanding their biology and potential therapeutic applications.
Used in Hematopoietic Progenitor Cell Research:
Protamine sulfate is used for the transduction of lentiviruses into human embryonic 293T cells and the delivery of lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells. This enables the study of gene function and the development of novel therapeutic strategies for blood disorders and other related diseases.

Biochem/physiol Actions

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.

Veterinary Drugs and Treatments

Protamine is used in all species for the treatment of heparin overdosage when significant bleeding occurs. While protamine will neutralize the anti-thrombin effects of low molecular weight heparins (e.g., dalteparin or enoxaparin), it does not completely inhibit their anti-Xa activity. Laboratory animal studies however, shows it does improve microvascular bleeding associated with LMWH overdoses. Protamine has been suggested for use for Bracken Fern toxicity in ruminants (see Doses).