mandelonitrile lyase
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- Company Profile
1. Principle.
Abbreviations used:
ML = Mandelonitrile Lyase
HCN = Hydrogen Cyanide
CONDITIONS: T = 25°C, pH = 5.4, A275nm, Light path = 1 cm
2. How to reaction ?
REAGENTS:
A. 100 mM Sodium Acetate Buffer(Prepare 100 ml in deionized water using Sodium Acetate Trihydrate, Sigma Prod. No. S-8625. Adjust to
pH 5.4 at 25°C with 1 M HCl.)
B. 95% (v/v) Ethanol
(Prepare 10 ml in deionized water using 200 Proof USP Ethyl Alcohol, available from Quantum Chemical Company.)
C. 168 mM DL-Mandelonitrile Solution (Substrate) (Prepare 10 ml in cold 95% Ethanol using DL-Mandelonitrile, Sigma Prod. No. M-7010. PREPAREFRESH.)
D. 0.1% (w/v) Bovine Serum Albumin Solution (Enzyme Diluent) (Prepare 10 ml in cold deionized water using Albumin,
Bovine, Sigma Prod. No. A-6003.)
E. Mandelonitrile Lyase Enzyme Solution (Immediately before use, prepare a solution containing 1 - 2 units/ml of Mandelonitrile Lyase in cold
Verified Supplier
JiangXi Keyuan Biopharma Co., LTD.
- Country: China (Mainland)
- Year Established: 2002
- Business License:
- Business type: Manufacturers
- Integral:
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