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mandelonitrile lyase

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    1. Principle.

     

    Abbreviations used:
    ML = Mandelonitrile Lyase
    HCN = Hydrogen Cyanide
    CONDITIONS: T = 25°C, pH = 5.4, A275nm, Light path = 1 cm

     

    2. How to reaction ?

     

    REAGENTS:
    A. 100 mM Sodium Acetate Buffer(Prepare 100 ml in deionized water using Sodium Acetate Trihydrate, Sigma Prod. No. S-8625. Adjust to
    pH 5.4 at 25°C with 1 M HCl.)
    B. 95% (v/v) Ethanol
    (Prepare 10 ml in deionized water using 200 Proof USP Ethyl Alcohol, available from Quantum Chemical Company.)
    C. 168 mM DL-Mandelonitrile Solution (Substrate) (Prepare 10 ml in cold 95% Ethanol using DL-Mandelonitrile, Sigma Prod. No. M-7010. PREPAREFRESH.)
    D. 0.1% (w/v) Bovine Serum Albumin Solution (Enzyme Diluent) (Prepare 10 ml in cold deionized water using Albumin,
    Bovine, Sigma Prod. No. A-6003.)
    E. Mandelonitrile Lyase Enzyme Solution (Immediately before use, prepare a solution containing 1 - 2 units/ml of Mandelonitrile Lyase in cold

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