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pyrene fluorescence

pyrene fluorescence is a potential tool for estimation of short-range lateral mobility in membranes of living renal epithelial cells
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    Pyrene is a highly sensitive fluorophore, and pyrene fluorescence is a potential tool for estimation of short-range lateral mobility in membranes of living renal epithelial cells.

     

    The pyrene fluorescence has been found to correlate with membrane fluidity. In Modin-Darby canine kidney cells, successful fluorescent labeling was obtained with p-cholesterol, p-sulfonamidethyl-TMA, p-trimethylammonium (TMA), p-propanoate, p-buty-rate, or p-dodecanoate. Among them, the labeling with p-cholesterol was most stable. The whole cell cholesterol content did not notably increase after loading p-cholesterol and the probe incorporated was not degraded. The E/M ratio changed reproducibly and significantly by temperature change, a standard method to alter membrane fluidity. Thus, the E/M ratio of p-cholesterol fluorescence is suggested to be a potential parameter of membrane fluidity in living renal epithelial cells and might be useful in investigating the relationship between the physical state of the membrane and epithelial function.

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