Characterization of a novel D-LYXOSE (cas 1114-34-7) isomerase from Thermoflavimicrobium dichotomicum and its application for D-mannose production

Add time:08/13/2019    Source:sciencedirect.com

d-Mannose is the aldose isomer of d-fructose and displays unique physiological functions and health applications. As a result, it has attracted increasing interest from the public. Because of its wide substrate specificity, D-LYXOSE (cas 1114-34-7) isomerase (D-LI) has been applied to d-mannose bioproduction. In this article, the Thermoflavimicrobium dichotomicum D-LI encoding gene was cloned and overexpressed in Escherichia coli BL21(DE3). The novel protein T. dichotomicum D-LI was identified and characterized, and its maximum enzyme activity was exhibited at pH 7.5 (phosphate buffer) and 60 °C in the presence of Mn2+. Similar to other D-LIs, T. dichotomicum D-LI formed a homodimeric structure and showed strict conservation of the metal coordination and substrate binding sites. The D-LI half-life (t1/2) was 9.91 h and 3.05 h at 55 and 60 °C, respectively, and its melting temperature (Tm) was 72.85 °C. T. dichotomicum D-LI displayed the highest specific activity towards d-lyxose among the substrates tested, followed by d-mannose. It also efficiently converted d-fructose to d-mannose, and the equilibrium ratio between d-mannose and d-fructose during T. dichotomicum D-LI activity was 25:75. From 500 g/L d-fructose, 110.5 g/L d-mannose was produced after 6 h, suggesting that T. dichotomicum D-LI might provide an alternative for d-mannose production.

We also recommend Trading Suppliers and Manufacturers of D-LYXOSE (cas 1114-34-7). Pls Click Website Link as below: cas 1114-34-7 suppliers

Prev:Ascorbic to tartaric acid conversion in grapevines
Next:Efficient biotransformation of d-fructose to d-mannose by a thermostable D-LYXOSE (cas 1114-34-7) isomerase from Thermosediminibacter oceani)