Cloning, characterization and transmission blocking potential of midgut CARBOXYPEPTIDASE A (cas 11075-17-5) in Anopheles stephensi

Add time:07/18/2019    Source:sciencedirect.com

Transmission-blocking vaccines (TBV) interrupt malaria parasite transmission and hence form an important component for malaria eradication. Mosquito midgut exopeptidases such as aminopeptidase N & carboxypeptidase B have demonstrated TBV potential. In the present study, we cloned and characterized CARBOXYPEPTIDASE A (cas 11075-17-5) (CPA) from the midgut of an important malarial vector, Anopheles stephensi. ClustalW amino acid alignment and in silico 3-dimensional structure analysis of CPA predicted the presence of active sites involved in zinc and substrate binding that are conserved among all the known mosquito species. Real-time PCR analysis demonstrated that CPA is predominantly expressed in the midgut throughout the mosquito life cycle and that this gene is significantly elevated in P. berghei-infected mosquitoes compared to uninfected blood-fed controls. The high midgut CPA activity correlated with the prominent mRNA levels observed. Peptide-based anti-CPA antibodies were raised that cross-reacted specifically to ∼48 kDa and ∼37 kDa bands, which correspond to zymogen and active forms of CPA. Further, the addition of CPA-directed antibodies to P. berghei-containing blood meal significantly reduced the mosquito infection rate in the test group compared to control and blocked the parasite development in the midgut. These results support further development of A. stephensi CPA as a candidate TBV.

We also recommend Trading Suppliers and Manufacturers of CARBOXYPEPTIDASE A (cas 11075-17-5). Pls Click Website Link as below: cas 11075-17-5 suppliers

Prev:Peptide-modified nanochannel system for carboxypeptidase B activity detection
Next:Application of CARBOXYPEPTIDASE A (cas 11075-17-5) to a baking process to mitigate contamination of wheat flour by ochratoxin A)