191723-64-5

Basic information

• Product Name: H-HIS-AMC
• Synonyms: H-HIS-AMC;L-HISTIDINE 7-AMIDO-4-METHYLCOUMARIN;L-histidine 7-amindo-4-methylcoumarin;H-L-His-AMC;H-His-AMC Trifluoroacetate salt;H-His-AMC.TFA;(alphaS)-alpha-Amino-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-1H-imidazole-5-propanamide;L-His-AMC·TFA
• CAS NO: 191723-64-5
• Molecular Formula: C16H16N4O3
• Molecular Weight: 312.32
• Product Categories: Other enzyme inhibitors and activators
• Mol File: 191723-64-5.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 696 °C at 760 mmHg
• Flash Point: 374.8 °C
• Appearance: /
• Density: 1.413 g/cm³
• Storage Temp.: -15°C
• CAS DataBase Reference: H-HIS-AMC(CAS DataBase Reference)
• NIST Chemistry Reference: H-HIS-AMC(191723-64-5)
• EPA Substance Registry System: H-HIS-AMC(191723-64-5)

Safety Data

• Hazardous Substances Data: 191723-64-5(Hazardous Substances Data)

Usage

Description

H-HIS-AMC, also known as L-Histidine 7-amido-4-methylcoumarin, is an enzyme substrate that is widely used in various fields due to its versatility and unique properties. It is an off-white to white powder that can be linked to the carboxy-terminus of any amino acid or peptide, making it a valuable tool for studying enzyme reactions. The low basicity of the amino group in H-HIS-AMC ensures that it is not subject to acid-base equilibrium at physiological pH, allowing for more reliable and accurate assays.

Uses

Used in Microbiology:
H-HIS-AMC is used as an enzyme substrate for studying the activity of glycosidases, phosphatases, and esterases in microbiological research. Its versatility and compatibility with most enzymes make it a preferred choice for investigating enzyme kinetics and function.
Used in Food and Water Analysis:
In the food and water industry, H-HIS-AMC is employed as an enzyme substrate for detecting and quantifying the presence of specific enzymes, which can help in assessing the quality and safety of these products.
Used in Environmental Testing:
H-HIS-AMC is used as an enzyme substrate in environmental testing to monitor the presence and activity of various enzymes, which can provide valuable information about the health of ecosystems and the potential presence of contaminants.
Used in ELISA Testing:
H-HIS-AMC is used as a fluorophore in enzyme-linked immunosorbent assay (ELISA) tests, allowing for the detection and quantification of specific antibodies or antigens in biological samples.
Used in Clinical Enzyme Assays:
In clinical settings, H-HIS-AMC is used as an enzyme substrate for assays involving peptidases, which can help in diagnosing and monitoring various diseases and conditions related to enzyme activity.
Overall, H-HIS-AMC is a versatile and valuable tool in various industries, including microbiology, food and water analysis, environmental testing, ELISA testing, and clinical enzyme assays, due to its unique properties and compatibility with a wide range of enzymes.