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Test compounds were added at varied concentrations and reac-
tions were initiated by the addition of 100 L of a substrate solu-
l
tion consisting of 0.055 mg/mL (Z-Lys-SBzl) (Bachem) and 24 mg/
mL DTNB. Reactions were incubated for 5 min at room tempera-
ture prior to measuring the change in OD405 nm for 10 min at
21-s intervals with a PowerwaveX (Bio-Tek Instruments) micro-
plate reader.
6.8. Thrombin
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Thrombin assay reactions were performed in a 200-lL mixture
containing (1 U/mL) thrombin (Sigma Chemicals), in an assay buf-
fer containing 100 mM HEPES, pH 7.5, 10 mM CaCl2, and 1%
dimethylsulfoxide (DMSO). Test compounds were added at varied
concentrations and reactions were initiated by the addition of
Na-Benzoyl-Phe-Val-Arg-p-Nitroanalide (Sigma Chemicals) at a fi-
nal concentration of 1 mM. Reactions were incubated for 5 min at
room temperature prior to measuring the change in OD405 nm for
10 min at 21-s intervals with a PowerwaveX (Bio-Tek Instruments)
microplate reader.
6.9. Trypsin
Trypsin assay reactions were performed in a 200-
lL mixture
containing (1 g/mL) trypsin (Sigma Chemicals), in an assay buffer
l
containing 200 mM triethylanolamine (TEA), pH 7.8, 10 mM CaCl2,
and 1% dimethylsulfoxide (DMSO). Test compounds were added at
varied concentrations and reactions were initiated by the addition
of Na-Benzoyl-L-Arginine-p-Nitroanalide (L-BAPNA, HCl) (Sigma
Chemicals) at a final concentration of 0.05 mg/mL. Reactions were
incubated for 5 min at room temperature prior to measuring the
change in OD405 nm for 10 min at 21-s intervals with a Powerwa-
veX (Bio-Tek Instruments) microplate reader.
Acknowledgments
The authors thank Drs. Charlie Bugg and Claude Bennett for
their encouragement throughout this work. The authors also ex-
press appreciation to Dr. J. Robert Piper for his assistance and
Mrs. Linda Kay First for preparation of this manuscript.
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