Communication
ChemComm
miR-34a function, which could be restored upon light irradia-
tion to remove biotin. Photolysis reaction of PA-miR-34a to
liberate miR-34a completed within two minutes’ irradiation,
which is rapid enough for control of miR-34a function in living
cells. By introducing PA-miR-34a into HeLa cells, we demonstrated
successful isolation and confirmation of miR-34a targets. Moreover,
we achieved spatiotemporal regulation of miR-34a target expression
and light-inducible cell apoptosis. Overall, this study provides a
practical tool for identification of miRNA targets and light-control
of miRNA target expression. Using PA-miRNA to identify unknown
miRNA targets and investigate the corresponding miRNA function
is now underway in our group.
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We would like to acknowledge the financial support from
the National Natural Science Foundation of China (21877058,
2017, 8, 15162; (c) F. Schafer, J. Wagner, A. Knau, S. Dimmeler and
21672103, 91753116, and 21572102) and the Fundamental
A. Heckel, Angew. Chem., Int. Ed., 2013, 52, 13558–13561.
Research Funds for the Central Universities (020514380137 10 (a) X. Xiao, X. Wang, Y. Wang, T. Yu, L. Huang, L. Chen, J. Li,
C. Zhang and Y. Zhang, Chem. – Eur. J., 2018, 24, 2277–2285;
and 020514380133).
(
b) M. Yamakuchi, M. Ferlito and C. J. Lowenstein, Proc. Natl. Acad.
Sci. U. S. A., 2008, 105, 13421–13426.
1 (a) Y. Ji, J. Yang, L. Wu, L. Yu and X. Tang, Angew. Chem., Int. Ed.,
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Conflicts of interest
2
016, 55, 2152–2156; (b) J. M. Govan, R. Uprety, M. Thomas,
H. Lusic, M. O. Lively and A. Deiters, ACS Chem. Biol., 2013, 8,
272–2282.
There are no conflicts to declare.
2
1
1
2 E. Slabakova, Z. Culig, J. Remsik and K. Soucek, Cell Death Dis.,
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3 G. Misso, M. T. Di Martino, G. De Rosa, A. A. Farooqi, A. Lombardi,
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