10191-18-1 Usage
Description
2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate, also known as BES, is a zwitterionic buffer used in biochemistry and molecular biology research. It is a secondary standard biochemical buffer with a useful pH range of 6.4 to 7.8. BES is known for its ability to induce cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes and acts as a biobuffer in various applications.
Uses
Used in Diagnostic Assay Manufacturing Industry:
2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate is used as a buffer to maintain the pH of solutions in biological experiments, which is crucial for the diagnostic assay manufacturing industry.
Used in Transfection Systems:
In the field of molecular biology, 2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate is used as a component of an improved transfection system for the stable transformation of cells with plasmid DNA. The slow formation of calcium phosphate-DNA complex and its gradual precipitation onto cells contribute to the high efficiency of stable transformations.
Used in Binding Assays:
2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate is used as a binding buffer in modified Eagle's medium during the binding assay of human melanoma cells, facilitating the study of cell interactions.
Used in Self-Assembly Investigations:
In the field of materials science, 2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate serves as a biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers, providing insights into the formation and properties of these materials.
Used in Molecular Biology Research:
2-[Bis(2-hydroxyethyl)ammonio]ethanesulfonate is used as a buffer in molecular biology research to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids and to determine the action of interfering factors in the bacterial endotoxins test.
Flammability and Explosibility
Notclassified
Purification Methods
Crystallise BES from aqueous EtOH. [Beilstein 4 IV 3290.]
Check Digit Verification of cas no
The CAS Registry Mumber 10191-18-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,1,9 and 1 respectively; the second part has 2 digits, 1 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 10191-18:
(7*1)+(6*0)+(5*1)+(4*9)+(3*1)+(2*1)+(1*8)=61
61 % 10 = 1
So 10191-18-1 is a valid CAS Registry Number.
InChI:InChI=1/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
10191-18-1Relevant articles and documents
Hydrogen ion buffers for biological research.
Good,Winget,Winter,Connolly,Izawa,Singh
, p. 467 - 477 (1966)
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A new strategy for the synthesis of taurine derivatives using the 'safety-catch' principle for the protection of sulfonic acids
Seeberger, Sonja,Griffin, Roger J.,Hardcastle, Ian R.,Golding, Bernard T.
, p. 132 - 138 (2008/03/14)
The safety-catch principle has been applied for the development of a new method for protecting sulfonic acids. 2,2-Dimethylsuccinic acid was reduced to 2,2-dimethylbutane-1,4-diol, which was selectively silylated to give 4-(tert-butyldiphenylsilanyloxy)-2,2-dimethylbutan-1-ol. Reaction of the latter compound with 2-chloroethanesulfonyl chloride in the presence of triethylamine afforded 4-(tert-butyldiphenylsilyloxy)-2,2-dimethylbutyl ethenesulfonate directly. The ethenesulfonate underwent Michael-type addition with secondary amines to give protected derivatives of taurine (2-aminoethanesulfonic acid). Deprotection was achieved on treatment with tetrabutylammonium fluoride, whereby cleavage of the silicon-oxygen bond led to an intermediate alkoxide that immediately cyclised to 2,2-dimethyltetrahydrofuran with liberation of a sulfonate. Pure sulfonic acids were obtained from the crude product by ion exchange chromatography on a strongly basic resin, which was eluted with aqueous acetic acid. The method developed should be generally applicable to the protection of sulfonic acids and is amenable to a multiparallel format. This journal is The Royal Society of Chemistry.
Isolation of nucleic acids
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, (2008/06/13)
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
