130-22-3 Usage
Description
Alizarin Red S is an organic sodium salt with the chemical structure of 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate as the counterion. It is a yellow-orange powder that is soluble in Cellosolve, slightly soluble in ethanol and acetone, and soluble in concentrated sulfuric acid, changing color from orange to pale yellow upon dilution. Alizarin Red S is commonly used to stain embryo skeletons in cleared whole mounts, typically of small mammals.
Uses
Used in Biochemical Assays:
Alizarin Red S is used as a biochemical assay to determine the presence of calcium. It is also used in a biochemical assay to determine the presence of calcific deposition by cells of an osteogenic lineage.
Used in Pharmaceutical Formulations:
Alizarin Red S sodium salt is used as a chromogenic agent for the colorimetric determination of dothiepin hydrochloride in pharmaceutical formulations. Additionally, it is used as an ion-pair reagent for the spectrophotometric assay of fexofenadine in pharmaceuticals.
Used as a Colorometric pH Indicator:
Alizarin Red S acts as a colorometric pH indicator in the range of 3.8-5.4.
Used in Dye Industry:
Alizarin Red S is used in the dye industry for various applications, such as producing dark blue shades with chromium, brilliant yellow-red shades with aluminum, and as a component of Claybank powder.
Standard:
Alizarin Red S has various standard ratings for different properties, such as ironing fastness (ISO 3, AATCC 3-4), light fastness (ISO 6, AATCC 6-7), fulling (ISO 4-5, AATCC 3), perspiration fastness (ISO 4-5, AATCC 5), soaping (ISO 5, AATCC 4), water (ISO 5), alkali (ISO 5), and acid (ISO 4).
Preparation
1,2-Dihydroxyanthracene-9,10-dione with fuming sulfuric acid sulphonate, and translated into Sodium salt.
Biochem/physiol Actions
Alizarin Red S is used for the staining of cartilage and bone. It reacts with calcium, thereby helping in the diagnosis of calcium deposits. It is also used to study carbohydrate–boronic acid interactions.
Standard
Ironing Fastness
Alkali
Acid
ISO
3
AATCC
3-4
Check Digit Verification of cas no
The CAS Registry Mumber 130-22-3 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,3 and 0 respectively; the second part has 2 digits, 2 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 130-22:
(5*1)+(4*3)+(3*0)+(2*2)+(1*2)=23
23 % 10 = 3
So 130-22-3 is a valid CAS Registry Number.
InChI:InChI=1/C14H8O7S.Na.H2O/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;;/h1-5,17-18H,(H,19,20,21);;1H2/q;+1;/p-1
130-22-3Relevant articles and documents
Alizarin red S-zinc(II) fluorescent ensemble for selective detection of hydrogen sulphide and assay with an H2S donor
Kaushik, Rahul,Kumar, Pawan,Ghosh, Amrita,Gupta, Neha,Kaur, Davinder,Arora, Saroj,Jose, D. Amilan
, p. 79309 - 79316 (2015)
A new Alizarin Red S based fluorescent probe ARS-Zn(ii) has been reported for the detection of H2S in aqueous buffer solution. In the presence of zinc ions, ARS displayed an increase in fluorescence intensity through the formation of an ARS-Zn(ii) ensemble. The addition of H2S to the ensemble caused the disassembly of the metal and ARS, which restored the fluorescence intensity of ARS. This sensing ensemble exhibited H2S-selectivity over other biothiols and biologically relevant analytes. The calculated detection limit of ARS-Zn(ii) with H2S was 92 nM. ARS-Zn(ii) also detected H2S in serum under physiological conditions. Moreover, as a practical application, it could be used for the real time monitoring of H2S released from the H2S-donor molecule benzoic (methyl carbonic) dithioperoxyanhydride and also applied to live cell imaging. The reported ensemble ARS-Zn(ii) has advantages like ready availability, high selectivity with good sensitivity, fast response time, higher excitation (520 nm) and emission (625 nm) wavelengths and real time fluorescence assay with H2S donors.