1476-53-5 Usage
Description
NOVOBIOCIN SODIUM SALT, also known as Albamycin, is a potent antibiotic derived from the Streptomyces spheroides bacteria. It is characterized by its crystalline structure and acts as an inhibitor of bacterial DNA gyrase and eukaryotic DNA topoisomerase. Additionally, it has been shown to inhibit retrovirus RNA-dependent DNA-polymerase. NOVOBIOCIN SODIUM SALT is more bioavailable than its calcium salt and parent acid, making it a preferred choice for various applications.
Uses
Used in Pharmaceutical Industry:
NOVOBIOCIN SODIUM SALT is used as an antibiotic for its antibacterial properties, particularly effective as an antistaphylococcal agent in the treatment of Methicillin-resistant Staphylococcus aureus (MRSA). It is also used to inhibit the activity of retroviral RNA-dependent DNA polymerase, bacterial DNA gyrase, and eukaryotic DNA topoisomerase, making it a versatile compound in the fight against bacterial and viral infections.
Used in Biotechnology Industry:
NOVOBIOCIN SODIUM SALT is used as a production aid for positively supercoiled plasmid DNA, which is essential in genetic engineering and molecular biology research. Its ability to inhibit DNA gyrase and topoisomerase II makes it a valuable tool in the creation and manipulation of plasmid DNA for various biotechnological applications.
Used in Research and Development:
Due to its inhibitory effects on DNA gyrase and topoisomerase II, NOVOBIOCIN SODIUM SALT is used in research and development for studying the mechanisms of DNA replication, transcription, and repair. It also aids in understanding the role of these enzymes in bacterial and eukaryotic cells, as well as their potential as targets for novel therapeutic agents.
Clinical Use
The action of novobiocin is largely bacteriostatic. Itsmode of action is not known with certainty, though it doesinhibit bacterial protein and nucleic acid synthesis. Studiesindicate that novobiocin and related coumarin-containingantibiotics bind to the subunit of DNA gyrase and possiblyinterfere with DNA supercoiling and energy transductionin bacteria. The effectiveness of novobiocin is confinedlargely to Gram-positive bacteria and a few strains of P. vulgaris.Its low activity against Gram-negative bacteria is apparentlybecause of poor cellular penetration.Although cross-resistance to other antibiotics is reportednot to develop with novobiocin, resistant S. aureus strainsare known. Consequently, the medical use of novobiocin isreserved for the treatment of staphylococcal infections resistantto other antibiotics and sulfas and for patients allergicto these drugs. Another shortcoming that limits the usefulnessof novobiocin is the relatively high frequency ofadverse reactions, such as urticaria, allergic rashes, hepatotoxicity,and blood dyscrasias.
Safety Profile
Poison by
intraperitoneal and subcutaneous routes.
Moderately toxic by ingestion. Mutation data
reported. An antibiotic with serious side
effects which include liver and blood
disease. It may also promote the
development of resistant strains of
staphylococcus. When heated to
decomposition it emits toxic fumes of NOx
and Na2O.
Purification Methods
The sodium salt has been recrystallised from MeOH, then dried at 60o/0.5mm. [Sensi et al, Anal Chem 29 1611 1957, Kaczka et al. J Am Chem Soc 78 4126 1956, Beilstein 18/8 IV 8125.]
Check Digit Verification of cas no
The CAS Registry Mumber 1476-53-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,4,7 and 6 respectively; the second part has 2 digits, 5 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 1476-53:
(6*1)+(5*4)+(4*7)+(3*6)+(2*5)+(1*3)=85
85 % 10 = 5
So 1476-53-5 is a valid CAS Registry Number.
InChI:InChI=1/C31H36N2O11.Na/c1-14(2)7-8-16-13-17(9-11-19(16)34)27(37)33-21-22(35)18-10-12-20(15(3)24(18)42-28(21)38)41-29-23(36)25(43-30(32)39)26(40-6)31(4,5)44-29;/h7,9-13,23,25-26,29,34-36H,8H2,1-6H3,(H2,32,39)(H,33,37);/q;+1/p-1/t23-,25+,26-,29-;/m1./s1
