2645-32-1 Usage
Description
PR-619 is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs) with a broad range of activity. It is highly useful for preserving ubiquitinated proteins during cell lysis and can be used to study the role of DUBs in intact cell systems.
Uses
Used in Biochemistry Research:
PR-619 is used as a component of lysis buffer for preserving ubiquitinated proteins during cell lysis. It helps to prevent the deubiquitination of proteins of interest, allowing for maximum preservation of ubiquitinated protein.
Used in Proteomics:
PR-619 is used as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells. This application aids in the study of protein ubiquitination and deubiquitination processes.
Used in Virology:
PR-619 is used as a pan-deubiquitinase inhibitor to study its effect on adeno-associated virus (AAV) transduction. This helps researchers understand the role of DUBs in viral processes.
Used in Immunology:
PR-619 is used in radioimmunoprecipitation assay (RIPA) buffer for ubiquitination assays. This application is crucial for investigating the ubiquitination of proteins in various biological processes.
Used in Organic Synthesis:
PR-619 is used in the preparation of thiazolopyridines and bisthiazolopyridines, which are important compounds in organic chemistry and drug development.
Biochem/physiol Actions
PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity.
References
1) Altun?et al. (2011),?Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes; Chem. Biol.,?18?1401
2) Seiberlich?et al. (2012),?The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: implications for neurodegenerative diseases; Biochim. Biophys. Acta,?1823?2057
Check Digit Verification of cas no
The CAS Registry Mumber 2645-32-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,6,4 and 5 respectively; the second part has 2 digits, 3 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 2645-32:
(6*2)+(5*6)+(4*4)+(3*5)+(2*3)+(1*2)=81
81 % 10 = 1
So 2645-32-1 is a valid CAS Registry Number.
2645-32-1Relevant articles and documents
Resonance-stabilized 1,2,3-dithiazolo-1,2,3-dithiazolyis as neutral π-radical conductors
Beer, Leanne,Reed, Robert W.,Brusso, Jaclyn L.,Cordes, A. Wallace,Haddon, Robert C.,Itkis, Mikhail E.,Kirschbaum, Kristin,MacGregor, Douglas S.,Oakley, Richard T.,Pinkerton, A. Alan
, p. 9498 - 9509 (2002)
Alkylation of the zwitterionic heterocycle 8-chloro-bis[1,2,3]dithiazolo[4,5-b:5′,4′-e]pyridine (CIBP) with alkyl triflates affords 8-chloro-4-alkyl-4H-bis[1,2,3]dithiazolo[4,5-b:-5′,4′-e]pyridin- 2-ium triflates [ClBPR]-[OTf] (R = Me, Et, Pr). Reduction of these salts with decamethylferrocene affords the corresponding ClBPR radicals as thermally stable crystalline solids. The radicals have been characterized in solution by cyclic voltammetry and EPR spectroscopy. Measured electrochemical cell potentials and computed (B3LYP/6-31G**) gas-phase disproportionation enthalpies are consistent with a low on-site Coulombic barrier U to charge transfer in the solid state. The crystal structures of ClBPR (R = Me, Et, Pr) have been determined by x-ray crystallography (at 293 K). All three structures consist of slipped π-stacks of undimerized radicals, with many close intermolecular S···S contacts. CIBPMe undergoes a phase transition at 93 K to a slightly modified slipped π-stack arrangement, the structure of which has also been established crystallographically (at 25 K). Variable-temperature magnetic and conductivity measurements have been performed, and the results interpreted in light of extended Hueckel band calculations. The room-temperature conductivities of ClBPR systems (σRT ≈ 10-5 to 10-6 S cm-1), as well as the weak 1D ferromagnetism exhibited by CIBPMe, are interpreted in terms of weak intermolecular overlap along the π-stacks. The latter is caused by slippage of the molecular plates, a feature necessitated by the steric size of the R and Cl groups on the pyridine ring.
