65-82-7 Usage
Description
N-Acetyl-L-methionine is a modified form of the amino acid methionine, where an acetyl group is attached to the nitrogen atom. It is known for its potential role in various biological processes and applications.
Uses
Used in Biomedical Research:
N-Acetyl-L-methionine is used as a research compound for studying metabolic phenotypes of standard and cold-stored platelets. This helps in understanding the changes in platelet metabolism and its implications in various conditions.
Used in Pharmaceutical Industry:
N-Acetyl-L-methionine is used as a pharmaceutical ingredient for its potential therapeutic applications. Its role in metabolic processes and its antioxidant properties make it a promising candidate for the development of drugs targeting various health issues.
Used in Cosmetic Industry:
N-Acetyl-L-methionine is used as an active ingredient in cosmetic products for its skin-friendly properties. It may help in maintaining skin health and providing antioxidant benefits, contributing to the overall well-being of the skin.
Used in Food Industry:
N-Acetyl-L-methionine is used as a food additive for its potential health benefits. It may be incorporated into dietary supplements or functional foods to provide additional nutritional support and promote overall health.
Identification Test
Take a sample of 250mg, get it dissolved it in 2.5ml isopropyl alcohol and get it diluted with water to 25ml. Take this liquid of 10ml and get it diluted with water to 100m1. Then take the liquid of 0.5,30 and 50μ1 after two dilutions. Drop it at the place with a distance of 2cm from the bottom edge of the thin-layer chromatographic plate. The plate is coated with silica gel (Namely, 20cm * 20cm Brinkman silica gel of 60.250nm, or the equivalent). In a sealed and balanced thin-layered chromatography expansion tank, The chromatography was splayed out by 10cm, and the developer was composed of n-butanol and the mixture of acetic acid and water in the proportion 75:20=20.
Dry the plate overnight. Then spray the chromatography plate with a new iodoplatinate solution mixed with 10 percent chloroplatinic acid of 3ml, water of 97ml and 6 percent potassium iodide solution of 100ml. The sample shall be formed - a separate color spot with a Rf value of 0.67~0.1
Content Analysis
Weigh accurately about 250mg sample and put it in a flask with glass stopper. Add 100ml water, 5g dipotassium hydrogen phosphate, 2g potassium dihydrogen phosphate and 2g potassium iodide. Get it fully mixed and dissolved, plus 0. 1mol/L iodine solution of 50.0m1. Then stopper it and blend it. Leave it stand for 30 minutes and titrate excess iodine with 0.1mol / L sodium thiosulfate. Meanwhile conduct blank titration experiment. Iodine solution per Ml 0.1mol/L equals to this product (C7H13NO3S) 9.563mg.
Toxicity Grade
Safe for food(FDA,§172.372,2000).
Acute Toxicity
Vein - mouse LD50: 435 mg / kg
Use Quantity
Account for 3.1% of the total protein content in food (Calculated by free L- methionine).
It shall not be used for infant food and food containing adding nitrite / nitrate (FDA, 172.3722000).
Safety Profile
Poison by intravenous route. When heated to decomposition emits toxic fumes of NOX,.
Purification Methods
Crystallise N-acetyl-L-methionine from Me2CO, H2O or EtOAc. Dry it in a vacuum over P2O5. Its solubility at 25o in H2O is 30.7%, and in Me2CO it is 29.5%. [Mitzi & Schueter Biochim Biophys Acta 27 168 1958, Birnbaum et al. J Biol Chem 194 455 1952, Beilstein 4 IV 3206.]
Check Digit Verification of cas no
The CAS Registry Mumber 65-82-7 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 6 and 5 respectively; the second part has 2 digits, 8 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 65-82:
(4*6)+(3*5)+(2*8)+(1*2)=57
57 % 10 = 7
So 65-82-7 is a valid CAS Registry Number.
InChI:InChI=1/C7H13NO3S/c1-5(9)8-6(7(10)11)3-4-12-2/h6H,3-4H2,1-2H3,(H,8,9)(H,10,11)/p-1/t6-/m0/s1
65-82-7Relevant articles and documents
Structure-activity relationship studies of dipeptide-based hepsin inhibitors with Arg bioisosteres
Kwon, Hongmok,Ha, Hyunsoo,Jeon, Hayoung,Jang, Jaebong,Son, Sang-Hyun,Lee, Kiho,Park, Song-Kyu,Byun, Youngjoo
supporting information, (2020/12/25)
Hepsin is a type II transmembrane serine protease (TTSP) associated with cell proliferation and overexpressed in several types of cancer including prostate cancer (PCa). Because of its significant role in cancer progression and metastasis, hepsin is an attractive protein as a potential therapeutic and diagnostic biomarker for PCa. Based on the reported Leu-Arg dipeptide-based hepsin inhibitors, we performed structural modification and determined in vitro hepsin- and matriptase-inhibitory activities. Comprehensive structure-activity relationship studies identified that the p-guanidinophenylalanine-based dipeptide analog 22a exhibited a strong hepsin-inhibitory activity (Ki = 50.5 nM) and 22-fold hepsin selectivity over matriptase. Compound 22a could be a prototype molecule for structural optimization of dipeptide-based hepsin inhibitors.
Synthesis method of (2S)-2-(acetamino)-4-(methylsulfinyl)butyric acid
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Paragraph 0019, (2019/01/08)
The invention relates to a synthesis method of (2S)-2-(acetamino)-4-(methylsulfinyl)butyric acid and mainly aims to solve the technical problems of high cost, high pollution, numerous by-products andharm to mass production of the existing synthesis method. The method comprises the following steps: (1) dissolving solid L-methionine in a sodium carbonate aqueous solution, dropwise adding liquid acetic anhydride, stirring for reacting, filtering and washing unreacted acetic anhydride in filtrate by using a mixed solution of ethyl acetate and petroleum ether, adding an extraction agent which is ethyl acetate into a water phase, acidizing by using solid citric acid, layering, washing, drying and carrying out reduced-pressure distillation to obtain an intermediate which is Nalpha-acetyl-L-methionine; and (2) dissolving the intermediate which is Nalpha-acetyl-L-methionine in acetic acid, dropwise adding hydrogen peroxide with mass percentage concentration of 30%, stirring for reacting, concentrating an obtained product till the product is dry, adding alcohol and crystallizing to obtain the product which is (2S)-2-(acetamino)-4-(methylsulfinyl)butyric acid. The (2S)-2-(acetamino)-4-(methylsulfinyl) butyric acid is used as a raw material for synthesizing a polypeptide drug.
Peptide Tyrosinase Activators
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, (2015/06/10)
Peptides that increase melanin synthesis are provided. These peptides include pentapeptides YSSWY, YRSRK, and their variants. The peptides may activate the enzymatic activity of tyrosinase to increase melanin synthesis. The pharmaceutical, cosmetic, and other compositions including the peptides are also provided. The methods of increasing melanin production in epidermis of a subject are provided where the methods include administering compositions comprising an amount of one or more peptides effective to increase the melanin production. The methods also include treating vitiligo or other hypopigmentation disorders with compositions including one or more peptides.