7365-45-9 Usage
Description
HEPES, also known as 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a widely used all-purpose buffer in biological research. It is a zwitterionic molecule effective as a buffer at pH 6.8 to 8.2 (pKa 7.55) and is typically used in cell culture at concentrations between 5mM to 30 mM. HEPES is water-soluble, atmosphere-independent, and is known for its ability to maintain a constant pH range for long-term experiments.
Uses
Used in Biological Research:
HEPES is used as a hydrogen ion buffer for maintaining a constant pH range in various biological experiments. It is particularly useful in cell culture applications, where it helps maintain physiological pH levels. The concentration used is typically between 10-50mmol/L, with 20mmol/L being a common concentration for achieving buffer ability in nutrient solutions.
Used in Cell Culture:
HEPES is used as a buffering component in the preparation of cell culture media. It is commonly found in solutions such as Hank's balanced salt solution, Dulbecco's modified eagle's medium, and no-glucose DMEM, which are used for the preparation of tissue slices.
Used in Tissue Culture:
HEPES buffered saline is a tissue culture buffer that helps maintain the pH of cell culture. It is particularly useful in in vitro experiments on Mg, where it helps control the pH of the surrounding environment.
Used in Conductance Measurements:
HEPES is used as a component of buffers in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Biological Activity
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Biological Activity
Multi purpose buffer used in biological research.
Purification Methods
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
Check Digit Verification of cas no
The CAS Registry Mumber 7365-45-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,3,6 and 5 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 7365-45:
(6*7)+(5*3)+(4*6)+(3*5)+(2*4)+(1*5)=109
109 % 10 = 9
So 7365-45-9 is a valid CAS Registry Number.
InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1
7365-45-9Relevant articles and documents
Preparation method of piperazine ethanesulfonic acid derivative (by machine translation)
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Paragraph 0048-0087, (2020/02/06)
The reaction is carried out in such a manner that the reaction proceeds relatively, more thoroughly by reducing the side, reaction of the reaction system, in a manner such that the reaction proceeds relatively more thoroughly from, the system to 2 - a certain extent 2 - by reducing the reaction proceeds, to relatively more thoroughly; NaOH, NaCl, and. (by machine translation)
Method for preparing high-purity 4-hydroxyethylpiperazine ethanesulfonic acid
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Paragraph 0034; 0035; 0047, (2017/07/19)
The invention belongs to the technical field of preparation of organic matters, and relates to a method for preparing high-purity 4-hydroxyethylpiperazine ethanesulfonic acid. The method sequentially comprises the following specific steps: preparation of 4-hydroxyethylpiperazine ethanesulfonate, acidizing, extraction for decolorizing and removing impurities, reverse extraction, nanofiltration and washing, and drying, so that the high-purity 4-hydroxyethylpiperazine ethanesulfonic acid is finally obtained. The 4-hydroxyethylpiperazine ethanesulfonic acid prepared with the method provided by the invention is high in purity; the operation process is simple and convenient; the production process is environmentally friendly; the purification cost is low; the yield is high; the method is suitable for industrial batch production.
Peptides for Treatment of Obesity
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, (2013/03/26)
The present invention relates to novel peptide compounds which are effective in modulating one or more melanocortin receptor types, to the use of the compounds in therapy, to methods of treatment comprising administration of the compounds to patients in need thereof, and to the use of the compounds in the manufacture of medicaments. The compounds of the invention are of particular interest in relation to the treatment of obesity as well as a variety of diseases or conditions associated with obesity.