9000-83-3

Basic information

• Product Name: ADENOSINE 5'-TRIPHOSPHATASE
• Synonyms: ADENOSINE 5'-TRIPHOSPHATASE;ATPASE;ATP PHOSPHOHYDROLASE;EC 3.6.1.3;ADENOSINE 5'-TRIPHOSPHATASE FROM RABBIT KIDNEY;ADENOSINE 5'-TRIPHOSPHATASE FROMPORCINE CEREBRAL C;ADENOSINE 5'-TRIPHOSPHATASE FROM DOG &;ADENOSINE-5'-TRIPHOSPHATASE FROM DOG KIDNEY, ~0.1 U/MG*
• CAS NO: 9000-83-3
• Molecular Formula: C10H16N5O13P3
• Molecular Weight: 0
• Mol File: 9000-83-3.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• CAS DataBase Reference: ADENOSINE 5'-TRIPHOSPHATASE(CAS DataBase Reference)
• NIST Chemistry Reference: ADENOSINE 5'-TRIPHOSPHATASE(9000-83-3)
• EPA Substance Registry System: ADENOSINE 5'-TRIPHOSPHATASE(9000-83-3)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9000-83-3(Hazardous Substances Data)

Usage

Description

ADENOSINE 5'-TRIPHOSPHATASE, commonly known as ATPase, is an enzyme that catalyzes the hydrolysis of adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and inorganic phosphate. This process is essential for various cellular functions, including energy transfer, signal transduction, and ion transport. ATPase plays a crucial role in maintaining cellular homeostasis and is involved in numerous physiological processes.

Uses

Used in Biochemical Research:
ATPase is used as a research tool for studying the mechanisms of ATP hydrolysis and its role in cellular processes. It is employed in various assays to investigate the activity and regulation of ATPases, as well as their interactions with other cellular components.
Used in Inhibition Assays:
ATPase, specifically from porcine cerebral cortex, is used as an enzyme source in inhibition assays to study the effects of different compounds on Na/K-ATPase activity. These assays help researchers understand the structure, function, and regulation of ATPases, as well as their potential as therapeutic targets for various diseases.
Used in Energy Metabolism Studies:
ATPase is used to investigate the role of ATP in cellular energy metabolism. By studying the hydrolysis of ATP to ADP and inorganic phosphate, researchers can gain insights into the regulation of energy production and consumption in cells.
Used in Ion Transport Research:
ATPase is involved in the active transport of ions across cellular membranes, a process that is essential for maintaining cellular homeostasis. ATPase is used in research to understand the mechanisms of ion transport and the role of ATPases in this process.
Used in Drug Discovery:
ATPase is a potential target for drug discovery, as its inhibition or modulation can have therapeutic effects in various diseases. Researchers use ATPase in screening assays to identify compounds that can modulate its activity, leading to the development of new drugs.

Biochem/physiol Actions

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .