Oriented covalent immobilization of recombinant protein A on the Glutaraldehyde (cas 111-30-8) activated agarose support

Add time:08/24/2019    Source:sciencedirect.com

High IgG-binding capacity of protein A affinity chromatography is crucial to its application in the antibody purification and autoantibody-associated disease treatment. An oriented immobilization strategy was used to covalently conjugate the recombinant protein A (rSpA) on the Glutaraldehyde (cas 111-30-8) activated agarose. By controlling the glutaraldehyde concentration, pH and reactivity time, one or two molecules of glutaraldehyde per primary amino group were anchored on agarose supports. The structure differences of activated supports were evaluated. Moreover, the 3D surface structure of B domain was modeled to explore the distribution of reactive and adsorptive groups. Compared with the monomeric glutaraldehyde agarose ([email protected]), the dimeric glutaraldehyde agarose ([email protected]) seems to be involved with more amino acid groups of rSpA during the immobilization. The leaked rSpA of 0.24 ng/mg IgG from [email protected]@rSpA was slightly lower than that of 0.36 ng/mg IgG from [email protected]@rSpA. However, [email protected] is more suitable for oriented immobilization of rSpA which endows the prepared adsorbents to higher IgG-binding capacity. When rSpA was immobilized on [email protected] at the low and high ionic strength, the maximum capacities from Langmuir model were 56.2 and 59.2 mg/g, respectively. The [email protected] provided shorter spacer arm compared with the [email protected], which contributed to the oriented immobilization of rSpA.

We also recommend Trading Suppliers and Manufacturers of Glutaraldehyde (cas 111-30-8). Pls Click Website Link as below: cas 111-30-8 suppliers

Prev:Immobilization and stabilization of different β-glucosidases using the Glutaraldehyde (cas 111-30-8) chemistry: Optimal protocol depends on the enzyme
Next:A sustainable solution for removal of Glutaraldehyde (cas 111-30-8) in saline water with visible light photocatalysis)