Gly-Arg-p-nitroanilide dihydrochloride

Base Information

• Chemical Name: Gly-Arg-p-nitroanilide dihydrochloride
• CAS No.: 125455-61-0
• Molecular Formula: C₁₄H₂₁N₇O₄*2ClH
• Molecular Weight: 424.287
• DSSTox Substance ID: DTXSID10585171
• Mol file: 125455-61-0.mol

Synonyms:Gly-Arg-p-nitroanilide dihydrochloride;2-[(2-aminoacetyl)amino]-5-(diaminomethylideneamino)-N-(4-nitrophenyl)pentanamide;hydrochloride;Gly-arg p-nitroanilide dihydrochloride;DTXSID10585171;Gly-Arg-p-nitroanilide dihydrochloride, unokinase and plasmin substrate;Glycyl-N~5~-(diaminomethylidene)-N-(4-nitrophenyl)ornithinamide--hydrogen chloride (1/1)

Chemical Property of Gly-Arg-p-nitroanilide dihydrochloride

Chemical Property:

• PSA: 195.43000
• LogP: 3.36980
• Storage Temp.: −20°C
• Hydrogen Bond Donor Count: 6
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 8
• Exact Mass: 387.1421799
• Heavy Atom Count: 26
• Complexity: 494

Purity/Quality:

98%min ^*data from raw suppliers

Gly-Arg-p-nitroanilide dihydrochloride unokinase and plasmin substrate ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: C1=CC(=CC=C1NC(=O)C(CCCN=C(N)N)NC(=O)CN)[N+](=O)[O-].Cl
• Description: H-Gly-Arg-pNA is a colorimetric substrate for thrombin. Thrombin preferentially binds to and cleaves the Gly-Arg (GR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity.

Technology Process of Gly-Arg-p-nitroanilide dihydrochloride

There total 2 articles about Gly-Arg-p-nitroanilide dihydrochloride which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 84.0%

Boc-Gly-Arg-pNA*HCl → 2HCl*H-Gly-Arg-pNA (125455-61-0)

Guidance literature:

With hydrogenchloride; In acetic acid; ethyl acetate; for 2h; Ambient temperature;

DOI:10.1016/0040-4020(95)00671-T

Reference yield:

L-arginine p-nitroanilide dihydrochloride (40127-11-5,75613-02-4) → 2HCl*H-Gly-Arg-pNA (125455-61-0)

Guidance literature:

Multi-step reaction with 2 steps

1: 75 percent / DIPEA / dimethylformamide / 2.5 h / Ambient temperature

2: 84 percent / 3.5 M HCl / ethyl acetate; acetic acid / 2 h / Ambient temperature

With hydrogenchloride; N-ethyl-N,N-diisopropylamine; In acetic acid; ethyl acetate; N,N-dimethyl-formamide;

DOI:10.1016/0040-4020(95)00671-T

upstream raw materials:

L-arginine p-nitroanilide dihydrochloride

Refernces