596-03-2 Usage
Description
4',5'-DIBROMOFLUORESCEIN is a synthetic, brilliant yellow-orange powder that exhibits unique solubility and fluorescence properties when dissolved in various solvents. It is slightly soluble in water, showing an orange color with weak yellow fluorescence, soluble in ethanol, displaying an orange color with green-yellow fluorescence, and soluble in acetone, presenting a peach blossom color with yellow fluorescence. Additionally, it is soluble in Tetrahydrofurfuryl alcohol. When in concentrated sulfuric acid, it appears as a red light yellow color, which turns to yellow brown upon dilution, accompanied by the formation of an orange precipitate. The dye's aqueous solution turns red upon the addition of sodium hydroxide. It demonstrates good dispersibility in glycerol and liquid paraffin.
Uses
Used in Dyes and Metabolites:
4',5'-DIBROMOFLUORESCEIN is used as a stain in the field of dyes and metabolites, particularly for environmental testing. Its absorption maximum of 450 nm makes it a valuable tool for detecting and analyzing various substances in environmental samples.
Used in Medicines and Cosmetics Coloring:
4',5'-DIBROMOFLUORESCEIN is utilized as a coloring agent in the pharmaceutical and cosmetics industries. Its vibrant color and fluorescence properties contribute to the visual appeal and effectiveness of various products, enhancing their marketability and consumer appeal.
Used in Environmental Testing:
In the environmental sector, 4',5'-DIBROMOFLUORESCEIN is employed as a stain for detecting and analyzing pollutants and other substances in environmental samples. Its unique fluorescence properties allow for the identification and quantification of specific contaminants, aiding in the assessment of environmental health and pollution control efforts.
Preparation
Fluorescein (C.I. Acid Yellow 73, C.I. 45350) in the sodium hydroxide solution two bromination, and??made into a free acid form.
Safety Profile
Poison by ingestion and
intravenous routes. An herbicide. When
heated to decomposition it emits highly
toxic fumes of NOx, CN-, and Br-. See also
NITRILES.
Purification Methods
Crystallise it from aqueous 30% EtOH. [Beilstein 19/6 V 462.]
Check Digit Verification of cas no
The CAS Registry Mumber 596-03-2 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,9 and 6 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 596-03:
(5*5)+(4*9)+(3*6)+(2*0)+(1*3)=82
82 % 10 = 2
So 596-03-2 is a valid CAS Registry Number.
596-03-2Relevant articles and documents
3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents
Busse, Madleen,Marciniszyn, Jaroslaw P.,Ferstl, Simone,Kimm, Melanie A.,Pfeiffer, Franz,Gulder, Tanja
supporting information, p. 4561 - 4566 (2021/02/12)
Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.
